| Country: | JPN |
| Type: | National |
| Route: | 129 |
| Map Custom: | yes |
| Length Km: | 31.7 |
| Length Ref: | [1] |
| Established: | 1963 |
| Direction A: | South |
| Terminus A: | in Hiratsuka, Kanagawa |
| Junction: | |
| Direction B: | North |
| Terminus B: | in Sagamihara, Kanagawa |
| Previous Type: | Route |
| Previous Route: | 128 |
| Next Type: | Route |
| Next Route: | 130 |
National Route 129 is a national highway of Japan connecting Hiratsuka and Sagamihara in Kanagawa Prefecture in the Kantō region of Japan. It has a total length of 31.7 km (19.7 mi).
It has junctions with National Route 16 in Sagamihara, National Routes 412 and 246 in Atsugi, Kanagawa, and National Routes 1 and 134 in Hiratsuka, Kanagawa. The route runs in a north to south direction and has junctions with some major expressways. These include, two interchanges with the Ken-Ō Expressway, Atsugi Interchange at the Tōmei Expressway and Odawara-Atsugi Road, and Atsugi-minami Interchange at the Shin-Tōmei Expressway.[2]
Route 129 was originally designated on 18 May 1953 from Yokohama to Chiba as a loop around Tokyo. This was redesignated (along with a section of Route 127) as Route 16 on 1 April 1963 and the current Route 129 was designated the same day.
The entire route is in Kanagawa Prefecture.