| James Estabrook | |
| Birth Date: | September 4, 1796 |
| Death Place: | Worcester, Massachusetts |
| Party: | Democratic |
| Spouse: | Almira Read |
| Title1: | Worcester County, Massachusetts sheriff |
| Term Start1: | 1851 |
| Term End1: | 1853 |
| Predecessor1: | John W. Lincoln |
| Successor1: | George W. Richardson |
| Term Start2: | 1848 |
| Term End2: | 1849 |
James Estabrook (September 4, 1796 – May 16, 1874) was a Worcester, Massachusetts grocer who served as the sheriff of Worcester County, Massachusetts from 1851 to 1853.
Estabrook was born to James and Betsey (Lovell) Estabrook in Holden, Massachusetts, on September 4, 1796.