| Birth Date: | 1991 6, df=yes |
| Birth Place: | Brisbane |
| Height: | 1.78 m |
| Weight: | 85 kg |
| Ru Position: | Fly-half |
| Youthyears2: | 2009−11 |
| Youthclubs1: | Sunnybank Rugby |
| Youthclubs2: | Brumbies Academy |
| Years1: | 2011−12 |
| Years2: | 2012−16 |
| Years3: | 2016−18 |
| Years4: | 2018−19 |
| Years5: | 2019−20 |
| Clubs1: | Hartpury |
| Clubs2: | Treviso |
| Clubs3: | San Donà |
| Clubs4: | Fiamme Oro |
| Clubs5: | Rovigo Delta |
| Apps2: | 52 |
| Apps3: | 32 |
| Apps4: | 16 |
| Apps5: | 5 |
| Points2: | 25 |
| Points3: | 282 |
| Points4: | 111 |
| Points5: | 12 |
| Repyears1: | 2012−2014 |
| Repyears2: | 2016− |
| Repteam1: | Emerging Italy |
| Repteam2: | Italy Seven |
| Repcaps1: | 6 |
| Repcaps2: | 43 |
| Reppoints1: | 31 |
| Reppoints2: | 171 |
| Ru Ntupdate: | 15 September 2017 |
James Ambrosini (born Brisbane, 5 June 1991) is an Australian-born Italian rugby union player. He plays for Rovigo Delta as a fly-half.
His father was born in Ari, Abruzzo, Italy and his mother is from Australia.[1] Due to his father, Ambrosini is eligible to play for the Italian national rugby union team.
He played for Benetton Treviso from 2012–13 Pro12 to 2015–16 Pro12 season.[2]
Ambrosini [3] signs with Amatori San Donà for the 2017–2018 season.[4]
He Played for Rovigo Delta for 2019–2020 season.[5]