| Country: | MYS |
| Type: | J |
| Route: | 33 |
| Direction A: | Northeast |
| Direction B: | Southwest |
| Terminus A: | Sungai Mati |
| Destinations: | Serom, Gerisek, Panchor, Bukit Kepong |
| Terminus B: | Bukit Serampang |
| Junction: | Federal Route 23 Jalan Panchor Jalan Bukit Serampang |
Jalan Bukit Gambir (Johor state route J33) is a major road in Johor, Malaysia. The roads is also a main route to North–South Expressway Southern Route via Bukit Gambir Interchange.
| Km | Exit | Junctions | To | Remarks | |
|---|---|---|---|---|---|
| Sungai Mati | North Tangkak Jementah Segamat Gunung Ledang North–South Expressway Southern Route Kuala Lumpur Melaka Johor Bahru Singapore Southwest Bandar Maharani (Muar) (Royal Town) Melaka | Junctions | |||
| Serom | |||||
| Bukit Gambir-NSE | North–South Expressway Southern Route North Kuala Lumpur Melaka Tangkak South Johor Bahru Yong Peng Pagoh | T-junctions with toll plaza | |||
| Bukit Gambir | |||||
| Jalan Panchor | Southeast Jalan Panchor Panchor Pagoh North–South Expressway Southern Route Kuala Lumpur Melaka Johor Bahru Singapore | T-junctions | |||
| Gerisek | |||||
| FELDA Bukit Serampang | |||||
| Bukit Serampang | Jalan Bukit Serampang West Sagil Jementah Tangkak Segamat East Bukit Kepong Labis | T-junctions |