| Played For: | Saskatoon Sheiks Vancouver Maroons Regina Capitals |
| Shoots: | Right |
| Height Ft: | 5 |
| Height In: | 11 |
| Weight Lb: | 155 |
| Birth Date: | 2 June 1895 |
| Birth Place: | Perth, Ontario, Canada |
| Career Start: | 1915 |
| Career End: | 1924 |
John Wesley Asselstine (June 2, 1895 – November 2, 1966[1]) was a Canadian professional ice hockey player. From 1922–1924 he played with the Saskatoon Sheiks and the Regina Capitals of the Western Canada Hockey League and the Vancouver Maroons of the Pacific Coast Hockey Association.