| Competition: | Israeli Premier League |
| Season: | 2000–01 |
| Winners: | Maccabi Haifa 6th title |
| Relegated: | Bnei Yehuda Hapoel Tzafririm Holon |
| League Topscorer: | Avi Nimni (25) |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 Israeli Premier League season had Maccabi Haifa win the title, while Bnei Yehuda and Tzafririm Holon were relegated.
The regular season took place from the first match on 12 August 2000 to the final match on 22 April 2001.The table was then divided into two groups, the upper and lower, which had six teams each. Then each team played the other five in their group once. The second stage was held between 27 April 2001 and 26 May 2001.
One team from Liga Leumit was promoted at the end of the previous season: Hapoel Tzafririm Holon. The three teams relegated were: Hapoel Kfar Saba, Maccabi Herzliya and Hapoel Jerusalem.
First 6 teams played in the top playoffs while the last 6 teams played in the bottom playoffs.
| Rank | Player | Club | Goals |
|---|---|---|---|
| 1 |  Avi Nimni | Maccabi Tel-Aviv | 25 |
| 2 | Motti Kakoun | Hapoel Petah Tikva | 19 |
| 3 | Viorel Tănase | Maccabi Netanya | 14 |
| Liron Vilner | Maccabi Netanya | 14 | |
| Itzik Zohar | Maccabi Netanya | 14 | |
| 6 | Yossi Benayoun | Maccabi Haifa | 13 |
| Rafi Cohen | Maccabi Haifa | 13 | |
| Amir Turgeman | Hapoel Haifa | 13 | |
| 9 | Alon Mizrahi | Beitar Jerusalem | 12 |
 Klimenti Tsitaishvili | Ironi Rishon LeZion | 12 |