4-Hydroxy-3-methylbut-2-enyl diphosphate reductase explained

4-Hydroxy-3-methylbut-2-enyl diphosphate reductase
Ec Number:	1.17.1.2
Cas Number:	512789-14-9

4-Hydroxy-3-methylbut-2-enyl diphosphate reductase (isopentenyl-diphosphate:NADP+ oxidoreductase, LytB, (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase, HMBPP reductase, IspH, LytB/IspH) is an enzyme in the non-mevalonate pathway. It acts upon (E)-4-Hydroxy-3-methyl-but-2-enyl pyrophosphate (or "HMB-PP").^{[1] [2] [3] [4] [5] [6]}

(1) isopentenyl diphosphate + NAD(P)⁺ + H₂O

\rightleftharpoons (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H⁺

(2) dimethylallyl diphosphate + NAD(P)⁺ + H₂O

\rightleftharpoons (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H⁺

4-Hydroxy-3-methylbut-2-enyl diphosphate reductase is an iron-sulfur protein that contains either a [3Fe-4S] or a [4Fe-4S] cluster.

Notes and References

1. Rohdich F, Hecht S, Gärtner K, Adam P, Krieger C, Amslinger S, Arigoni D, Bacher A, Eisenreich W . Studies on the nonmevalonate terpene biosynthetic pathway: metabolic role of IspH (LytB) protein . Proceedings of the National Academy of Sciences of the United States of America . 99 . 3 . 1158–63 . February 2002 . 11818558 . 122160 . 10.1073/pnas.032658999 . 2002PNAS...99.1158R . free .
2. Hintz M, Reichenberg A, Altincicek B, Bahr U, Gschwind RM, Kollas AK, Beck E, Wiesner J, Eberl M, Jomaa H . Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human gammadelta T cells in Escherichia coli . FEBS Letters . 509 . 2 . 317–22 . December 2001 . 11741609 . 10.1016/s0014-5793(01)03191-x . free .
3. On the reduction steps in the mevalonate independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in the bacterium Zymomonas mobilis . Charon, L. . Pale-Grosdemange, C. . Rohmer, M. . Tetrahedron Lett. . 1999 . 40 . 40 . 7231–7234 . 10.1016/s0040-4039(99)01531-2.
4. Röhrich RC, Englert N, Troschke K, Reichenberg A, Hintz M, Seeber F, Balconi E, Aliverti A, Zanetti G, Köhler U, Pfeiffer M, Beck E, Jomaa H, Wiesner J . Reconstitution of an apicoplast-localised electron transfer pathway involved in the isoprenoid biosynthesis of Plasmodium falciparum . FEBS Letters . 579 . 28 . 6433–8 . November 2005 . 16289098 . 10.1016/j.febslet.2005.10.037 . free .
5. Wolff M, Seemann M, Tse Sum Bui B, Frapart Y, Tritsch D, Garcia Estrabot A, Rodríguez-Concepción M, Boronat A, Marquet A, Rohmer M . Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein . FEBS Letters . 541 . 1–3 . 115–20 . April 2003 . 12706830 . 10.1016/s0014-5793(03)00317-x . free .
6. Gräwert T, Kaiser J, Zepeck F, Laupitz R, Hecht S, Amslinger S, Schramek N, Schleicher E, Weber S, Haslbeck M, Buchner J, Rieder C, Arigoni D, Bacher A, Eisenreich W, Rohdich F . IspH protein of Escherichia coli: studies on iron-sulfur cluster implementation and catalysis . Journal of the American Chemical Society . 126 . 40 . 12847–55 . October 2004 . 15469281 . 10.1021/ja0471727 .