Isoleucine N-monooxygenase explained
| Isoleucine N-monooxygenase |
| Ec Number: | 1.14.13.117 |
Isoleucine N-monooxygenase (CYP79D3, CYP79D4) is an enzyme with systematic name L-isoleucine,NADPH:oxygen oxidoreductase (N-hydroxylating).[1] [2] This enzyme catalyses the following chemical reaction
L-isoleucine + 2 O2 + 2 NADPH + 2 H+
(E)-2-methylbutanal oxime + 2 NADP
+ + CO
2 + 3 H
2O (overall reaction)
(1a) L-isoleucine + O2 + NADPH + H+
N-hydroxy-L-isoleucine + NADP
+ + H
2O
(1b) N-hydroxy-L-isoleucine + O2 + NADPH + H+
N,N-dihydroxy-L-isoleucine + NADP
+ + H
2O
(1c) N,N-dihydroxy-L-isoleucine
(E)-2-methylbutanal oxime + CO
2 + H
2O (spontaneous reaction)
Isoleucine N-monooxygenase is a heme-thiolate protein (P-450).
Notes and References
- Andersen MD, Busk PK, Svendsen I, Møller BL . Cytochromes P-450 from cassava (Manihot esculenta Crantz) catalyzing the first steps in the biosynthesis of the cyanogenic glucosides linamarin and lotaustralin. Cloning, functional expression in Pichia pastoris, and substrate specificity of the isolated recombinant enzymes . The Journal of Biological Chemistry . 275 . 3 . 1966–75 . January 2000 . 10636899 . 10.1074/jbc.275.3.1966 . free .
- Forslund K, Morant M, Jørgensen B, Olsen CE, Asamizu E, Sato S, Tabata S, Bak S . Biosynthesis of the nitrile glucosides rhodiocyanoside A and D and the cyanogenic glucosides lotaustralin and linamarin in Lotus japonicus . Plant Physiology . 135 . 1 . 71–84 . May 2004 . 15122013 . 429334 . 10.1104/pp.103.038059 .
