Isoflavonoid synthase explained

Isoflavonoid synthase
Ec Number:	1.14.13.136

2-hydroxyisoflavanone synthase (CYT93C, IFS, isoflavonoid synthase) is an enzyme with systematic name liquiritigenin,NADPH:oxygen oxidoreductase (hydroxylating, aryl migration).^{[1] [2] [3]} This enzyme catalyses the following chemical reactions:

liquiritigenin + O₂ + NADPH + H⁺

\rightleftharpoons 2,4',7-trihydroxyisoflavanone + H₂O + NADP⁺and

(2S)-naringenin + O₂ + NADPH + H⁺

\rightleftharpoons 2,4',5,7-tetrahydroxyisoflavanone + H₂O + NADP⁺

Isoflavonoid synthase requires cytochrome P450.

Notes and References

1. Hashim MF, Hakamatsuka T, Ebizuka Y, Sankawa U . Reaction mechanism of oxidative rearrangement of flavanone in isoflavone biosynthesis . FEBS Letters . 271 . 1–2 . 219–22 . October 1990 . 2226805 . 10.1016/0014-5793(90)80410-k . free .
2. Sawada Y, Kinoshita K, Akashi T, Aoki T, Ayabe S . Key amino acid residues required for aryl migration catalysed by the cytochrome P450 2-hydroxyisoflavanone synthase . The Plant Journal . 31 . 5 . 555–64 . September 2002 . 12207646 . 10.1046/j.1365-313x.2002.01378.x . free .
3. Sawada Y, Ayabe S . Multiple mutagenesis of P450 isoflavonoid synthase reveals a key active-site residue . Biochemical and Biophysical Research Communications . 330 . 3 . 907–13 . May 2005 . 15809082 . 10.1016/j.bbrc.2005.03.053 .