| Official Name: | Isabela Canton |
| Native Name: | Cantón Isabela |
| Settlement Type: | Canton |
| Flag Size: | 100px |
| Seal Size: | 80px |
| Subdivision Type: | Country |
| Subdivision Name: |  Ecuador |
| Subdivision Type1: | Province |
| Subdivision Name1: | Galápagos Province |
| Subdivision Type2: | Capital |
| Subdivision Name2: | Puerto Villamil |
| Area Total Km2: | 5,367 |
| Population As Of: | 2010 |
| Population Footnotes: | [1] |
| Population Total: | 1,780 |
| Population Density Km2: | auto |
| Coordinates: | -0.4724°N -90.6811°W |
Isabela Canton is a canton of Galápagos Province, Ecuador, consisting of the islands of Isabela Island, Darwin, Fernandina, Wolf, and some minor islets. Isabela is the largest island in the Galápagos, and well known for its beaches, bays and coastal lagoons with a wide variety of plants and birds. The capital of the canton is Puerto Villamil.
Isabela's human population has increased over the years, mainly during the 1970s, but the canton remains the least populated in the province, and among the least populated in Ecuador. Today fishing is the principal economic activity, but ecotourism and the production of handcrafts are becoming increasingly important.
Six volcanoes are found on the island of Isabela, of which two are over 1,700 meters, Wolf and Cerro Azul. The sites most commonly visited by tourists are Punta Garcia, to see the flightless cormorants; Volcán Alcedo for Land Tortoises; Tagus Cove for blue-footed boobies, flightless cormorants, Galápagos penguins, and marine iguanas; and Urbina Bay for sea turtles.