| Isaakio | |
| Name Local: | Ισαάκιο |
| Type: | community |
| Coordinates: | 41.363°N 26.539°W |
| Periph: | Eastern Macedonia and Thrace |
| Periphunit: | Evros |
| Municipality: | Didymoteicho |
| Municunit: | Didymoteicho |
| Population As Of: | 2021 |
| Population: | 316 |
Isaakio is a village of Evros regional unit in Greece, part of the municipality Didymoteicho.[1] It is located 5 kilometers east of Didymoteicho.[2] Its population according to the 2021 census was 316.