Iron–sulfur cluster biosynthesis explained
| Symbol: | Fe-S_biosyn |
| Fe-S_biosyn |
| Pfam: | PF01521 |
| Interpro: | IPR000361 |
| Prosite: | PDOC00887 |
| Scop: | 1nwb |
In biochemistry, the iron–sulfur cluster biosynthesis describes the components and processes involved in the biosynthesis of iron–sulfur proteins. The topic is of interest because these proteins are pervasive. The iron sulfur proteins contain iron–sulfur clusters, some with elaborate structures, that feature iron and sulfide centers. One broad biosynthetic task is producing sulfide (S2-), which requires various families of enzymes. Another broad task is affixing the sulfide to iron, which is achieved on scaffolds, which are nonfunctional. Finally these Fe-S cluster is transferred to a target protein, which then become functional.[1]
The formation of iron–sulfur clusters are produced by one of four pathways:[2]
- Nitrogen fixation (NIF) system, which is also found in bacteria that are not nitrogen-fixing.[3]
- Iron–sulfur cluster (ISC) system, in bacterial and mitochondria
- Sulfur assimilation (SUF) system, in plastids and some bacteria
In addition to those three systems, the so-called Cystosolic Iron–sulfur Assembly (CIA) is invoked for cytosolic and nuclear Fe–S proteins.
Mechanisms
The assembly of iron–sulfur clusters cluster begins with the production of the equivalent of a sulfur (sulfur atoms per se are not found in nature). The required sulfur atom is obtained from free cysteine by the action of so-called cysteine desulfurases. One prominent desulfurase is called IscS, a pyridoxal phosphate-dependent enzyme. The sulfur atom from the cysteine substrate is transferred to residue Cys-328 of IscS, forming a persulfide:
L-cysteine + [enzyme]-cysteine
L-alanine + [enzyme]-S-sulfanylcysteineThe persulfide
functional group R-S-S-H functions as a source of "inorganic sulfur" that will be incorporated into Fe-S clusters. Subsequently, IscS transfers this "extra" sulfur to IscU.
[4] In addition to IscS and IscU, bacterial Fe-S assembly requires IscA, an 11 kDa protein of uncertain function.
[5] The Suf system for iron–sulfur cluster biosynthesis is generally similar to the Isc system (and the Nif system). The analogy extends to the existence of SufA, SufS, and SufU. The Suf system operates with fewer chaperones.[1]
Notes and References
- Johnson DC, Dean DR, Smith AD, Johnson MK . Structure, function, and formation of biological iron–sulfur clusters . Annual Review of Biochemistry . 74 . 247–81 . 2005 . 15952888 . 10.1146/annurev.biochem.74.082803.133518 .
- Lill R . Function and biogenesis of iron-sulphur proteins . Nature . 460 . 7257 . 831–8 . August 2009 . 19675643 . 10.1038/nature08301 . 205217943 . 2009Natur.460..831L .
- Book: 10.1002/9781119951438.eibc2466 . FeS Cluster Assembly: NIF System in Nitrogen-Fixing Bacteria . Encyclopedia of Inorganic and Bioinorganic Chemistry . 2017. Santos PC, Dean DR . 1–13 . 978-1-119-95143-8 .
- Kato S, Mihara H, Kurihara T, Takahashi Y, Tokumoto U, Yoshimura T, Esaki N . Cys-328 of IscS and Cys-63 of IscU are the sites of disulfide bridge formation in a covalently bound IscS/IscU complex: implications for the mechanism of iron–sulfur cluster assembly . Proceedings of the National Academy of Sciences of the United States of America . 99 . 9 . 5948–52 . April 2002 . 11972033 . 122882 . 10.1073/pnas.082123599 . 2002PNAS...99.5948K . free .
- Cupp-Vickery JR, Silberg JJ, Ta DT, Vickery LE . Crystal structure of IscA, an iron–sulfur cluster assembly protein from Escherichia coli . Journal of Molecular Biology . 338 . 1 . 127–37 . April 2004 . 15050828 . 10.1016/j.jmb.2004.02.027 .
