Intron-encoded endonuclease I-SceI explained

Organism:	Saccharomyces cerevisiae S288C
Uniprot:	P03882
Intron-encoded endonuclease I-SceI
Symbol:	SCEI
Pdb:	1R7M

Intron-encoded endonuclease I-Sce I is a homing endonuclease. The enzyme is used in biotechnology as a meganuclease. It recognises an 18-base pair sequence TAGGGATAACAGGGTAAT and leaves a 4 base pair 3' hydroxyl overhang. It is a rare cutting endonuclease. Statistically an 18-bp sequence will occur once in every 6.9*10¹⁰ base pairs (a frequency of 1 in 4¹⁸). This sequence does not normally occur in a human or mouse genome.^[1]

Sources

I-SceI is coded by introns. It is present in the mitochondria of yeast Saccharomyces cerevisiae.

Notes and References

1. http://www.neb.com/nebecomm/products/productR0694.asp New England Biolabs site