Imperatoxin Explained

Imperatoxin I (IpTx) is a peptide toxin derived from the venom of the African scorpion Pandinus imperator.

There are two subtypes of this toxin:

• Imperatoxin A (activator): a peptide toxin which enhances the influx of Ca²⁺ from the sarcoplasmatic reticulum into the cell.
• Imperatoxin I (inhibitor): a peptide toxin which decreases the influx of Ca²⁺ from the sarcoplasmatic reticulum into the cell.

Imperatoxin A

The toxin comes from the venom of the African scorpion Pandinus imperator.^[1] The structure of IpTx_a consists of:

• 33 amino acids peptide (sequence GDCLPHLKRCKADNDCCGKKCKRRGTNAEKRCR, disulfide bonds Cys3-Cys17, Cys10-Cys21, Cys16-Cys32).
• the formula is C₁₄₈H₂₆₀N₅₈O₄₅S₆.
• shares the structure and function of the dihydropiridine receptor (DHPR). It corresponds to the II-III loop of the α1s subunit.
• three cysteine residues that form disulfide bridges to stabilize the three-dimensional structure.

The molecular weight of the toxin is 3.7 kDa.

IpTx_a acts on the Ryanodine receptors (RyR), which are intracellular Ca²⁺ release channels mainly known for their role in regulating Ca²⁺ release from the sarcoplasmatic reticulum of striated muscles.^[2] The peptide acts better on RyR type 1 than on type 3. RyR type 2 seems to be insensitive to IpTx_a.^[3]

The part of the peptide that looks like the II-III loop of the (DHPR) binds directly to RyR and enhances ryanodine binding to trigger Ca²⁺ release.^[3]

Imperatoxin I

The toxin comes from the venom of the African scorpion Pandinus imperator.^[1] The structure of IpTx_i consists of:

• Two polypeptides. A large subunit of 104 amino acids (sequence TMWGTKWCGSGNEATDISELGYWSNLDSCCRTHDHCDNIPSGQTKYGLTNEGKYTMMNCKCETAFEQCLRNVTGGMEGPAAGFVRKTYFDLYGNGCYNVQCPSQ) and a smaller one of 27 amino acids (sequence SEECPDGVATYTGEAGYGAWAINKLNG).
• Subunits are linked by a disulfide bond.
• Phospholipase A2 (PLA2) activity on the large subunit.

The molecular weight of the toxin is 15 kDa.

Like IpTx_a, IpTx_i acts on RyR.

When an action potential reaches the muscle, RyR channels open and Ca²⁺ becomes available in the cell to induce contraction. The presence of Ca²⁺ induces the large subunit of IpTx_i to hydrolyze the Sn2 fatty acyl bond from the membrane of the sarcoplasmatic reticulum. This process is executed by PLA2 activity. The freed fatty acids bind to the RyR itself or to a closely associated protein linked to gating. Binding of the RyR induces blocking of the channel. When the concentration of free fatty acids is low there will be an incomplete block of RyR; higher concentrations will give a complete block.^[4]

Because IpTx_i also works on the RyR channels of the heart muscles, it could potentially be used as a drug against arrhythmia. This has not yet been proven, and must be studied in vivo first.^[5]

Notes and References

1. 10.1073/pnas.89.24.12185 . Scorpion Toxins Targeted Against the Sarcoplasmic Reticulum Ca2+- Release Channel of Skeletal and Cardiac Muscle . 1992 . Proceedings of the National Academy of Sciences . 89 . 24 . 12185–9 . 2360882 . 1334561 . 1992PNAS...8912185V . 50723 . Hector H. . Valdivia . Mark S. . Kirby . W. Jonathan . Lederer . Roberto . Coronado. free .
2. 9234963 . 1997 . Franzini-Armstrong . C . Protasi . F . Ryanodine receptors of striated muscles: A complex channel capable of multiple interactions . 77 . 3 . 699–729 . Physiological Reviews . 10.1152/physrev.1997.77.3.699.
3. 10.1016/S0014-5793(01)03013-7 . Imperatoxin A (IpTx_a) from Pandinus imperator stimulates [³H]ryanodine binding to RyR3 channels . 2001 . Simeoni . Ilenia . Rossi . Daniela . Zhu . Xinsheng . Garcı́a . Jesus . Valdivia . Hector H. . Sorrentino . Vincenzo . FEBS Letters . 508 . 5–10 . 11707258 . 1. 24194429 .
4. 11886–94 . 10.1074/jbc.272.18.11886 . The Mechanism of Inhibition of Ryanodine Receptor Channels by Imperatoxin I, a Heterodimeric Protein from the Scorpion Pandinus imperator . 1997 . Zamudio . F. Z. . Journal of Biological Chemistry . 272 . 18 . 9115249 . Conde . R . Arévalo . C . Becerril . B . Martin . BM . Valdivia . HH . Possani . LD. free .
5. 937–44 . 10.1111/j.1745-7254.2007.00582.x . Ryanodine receptors as pharmacological targets for heart disease . 2007 . Santonastasi . Marco . Wehrens . Xander H T . Acta Pharmacologica Sinica . 28 . 7 . 17588328. free .