Ilaria Testa Explained

Ilaria Testa
Workplaces:Max Planck Institute for Multidisciplinary Sciences
KTH Royal Institute of Technology
Academic Advisors:Alberto Diaspro
Stefan Hell
Known For:RESOLFT, superresolution microscopy
Occupation:Physicist

Ilaria Testa is an Italian-born scientist who is a Fellow at the SciLifeLab in Stockholm and an Associate Professor at the Department of Applied Physics at the School of Engineering Science at the KTH Royal Institute of Technology.[1] She has made major contributions to advanced microscopy, particularly superresolution microscopy (RESOLFT, STED).

Education

Testa studied physics at University of Genoa in Italy and graduated with a M.Sc. in 2005. In 2009, she earned her Ph.D. in Biotechnology. During her Ph.D., she worked on quantitative methods in single-molecule biophysics and studied transitional states in fluorescent proteins.[2] After completing her thesis, supervised by Alberto Diaspro, she joined Stefan Hell's research group at the Max Planck Institute for Multidisciplinary Sciences in Göttingen, Germany as a postdoctoral researcher[3] where she had already spent part of her doctoral studies.

Career and research

At the Max Planck Institute for Multidisciplinary Sciences in Göttingen, Testa played a central role in establishing the superresolution technique RESOLFT, showing that superresolution microscopy can be realized with lower levels of light in living cells and tissues making it more attractive for its usage in the life sciences.[4] [5] [6] [7] [8]

In 2015, Testa was appointed Fellow at the SciLifeLab in Stockholm and assistant professor at the KTH Royal Institute of Technology. At the SciLifeLab, she set up the Laboratory for Advanced Optical BioImaging.[9] Recently, she was appointed associate professor.[10]

Testa and her team continue to develop further and use superresolution techniques such as STED and RESOLFT microscopy to understand the fundamental biological processes for health and diseases.[11] [12] [13]

Testa is a well-known microscopist who is an established member of the advanced microscopy community and is frequently invited on panels and as a keynote speaker at key conferences in the field.[14] [15] [16]

Awards and honors

Notes and References

  1. Web site: 2023-07-06. Ilaria Testa.
  2. Ilaria Testa. Vivien. Marx. August 1, 2018. Nature Methods. 15. 8. 557. 10.1038/s41592-018-0078-z. 30065372 . 256836501 . free.
  3. Web site: Interview: Staying focused. October 9, 2017. eLife.
  4. Diffraction-unlimited all-optical imaging and writing with a photochromic GFP. Tim. Grotjohann. Ilaria. Testa. Marcel. Leutenegger. Hannes. Bock. Nicolai T.. Urban. Flavie. Lavoie-Cardinal. Katrin I.. Willig. Christian. Eggeling. Stefan. Jakobs. Stefan W.. Hell. October 8, 2011. Nature. 478. 7368. 204–208. www.nature.com. 10.1038/nature10497. 21909116 . 2011Natur.478..204G . 2393728 .
  5. A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching. Tanja. Brakemann. Andre C.. Stiel. Gert. Weber. Martin. Andresen. Ilaria. Testa. Tim. Grotjohann. Marcel. Leutenegger. Uwe. Plessmann. Henning. Urlaub. Christian. Eggeling. Markus C.. Wahl. Stefan W.. Hell. Stefan. Jakobs. October 8, 2011. Nature Biotechnology. 29. 10. 942–947. www.nature.com. 10.1038/nbt.1952. 21909082 . 11858/00-001M-0000-0012-3E58-5 . 17238219 . free.
  6. Dual Channel RESOLFT Nanoscopy by Using Fluorescent State Kinetics. Ilaria. Testa. Elisa. D’Este. Nicolai T.. Urban. Francisco. Balzarotti. Stefan W.. Hell. January 14, 2015. Nano Letters. 15. 1. 103–106. CrossRef. 10.1021/nl503058k. 25423166 . 2015NanoL..15..103T .
  7. https://www.cell.com/neuron/pdf/S0896-6273(12)00719-2.pdf
  8. Web site: Pushing the Boundaries of Super-resolution Microscopy. www.youtube.com.
  9. Web site: TestaLab. www.testalab.org.
  10. Web site: KTH | Ilaria Testa. www.kth.se.
  11. Volumetric live cell imaging with three-dimensional parallelized RESOLFT microscopy. Andreas. Bodén. Francesca. Pennacchietti. Giovanna. Coceano. Martina. Damenti. Michael. Ratz. Ilaria. Testa. May 8, 2021. Nature Biotechnology. 39. 5. 609–618. www.nature.com. 10.1038/s41587-020-00779-2. 33432197 . 231585351 .
  12. Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems. Luciano A.. Masullo. Andreas. Bodén. Francesca. Pennacchietti. Giovanna. Coceano. Michael. Ratz. Ilaria. Testa. August 16, 2018. Nature Communications. 9. 1. 3281. 10.1038/s41467-018-05799-w. 30115928 . 6095837 . 2018NatCo...9.3281M .
  13. Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy. Francesca. Pennacchietti. Ekaterina O.. Serebrovskaya. Aline R.. Faro. Irina I.. Shemyakina. Nina G.. Bozhanova. Alexey A.. Kotlobay. Nadya G.. Gurskaya. Andreas. Bodén. Jes. Dreier. Dmitry M.. Chudakov. Konstantin A.. Lukyanov. Vladislav V.. Verkhusha. Alexander S.. Mishin. Ilaria. Testa. August 8, 2018. Nature Methods. 15. 8. 601–604. www.nature.com. 10.1038/s41592-018-0052-9. 29988095 . 256840689 .
  14. Web site: Seeing is Believing: Imaging the Molecular Processes of Life – Course and Conference Office. www.embl.org.
  15. Web site: Speakers – Rudolf-Virchow-Zentrum – Center for Integrative and Translational Bioimaging. www.uni-wuerzburg.de.
  16. Web site: Speakers – SMLMS 2023.
  17. Web site: Long-term molecular nanoscale imaging of neuronal function | MoNaLISA | Project | Fact sheet | H2020 | CORDIS | European Commission.
  18. Web site: ESP Young Investigator Award | European Society for Photobiology. www.photobiology.eu.
  19. Web site: Two KTH researchers receive ERC Consolidator Grants. KTH.
  20. Web site: Three SciLifeLab researchers receive ERC Consolidator Grants. December 17, 2020.
  21. Web site: List of Principal Investigators – LS domain.

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