Intersectin 2 Explained

Intersectin-2 is a protein that in humans is encoded by the ITSN2 gene.^{[1] [2] [3]}

This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis. Alternatively spliced transcript variants have been found for this gene that encode three distinct isoforms. Additional variants have been found but their full length nature has not been determined.^[4]

Interactions

ITSN2 has been shown to interact with Wiskott-Aldrich syndrome protein.

Further reading

• Andersson B, Wentland MA, Ricafrente JY, etal . A "double adaptor" method for improved shotgun library construction. . Anal. Biochem. . 236 . 1 . 107–13 . 1996 . 8619474 . 10.1006/abio.1996.0138 .
• Yu W, Andersson B, Worley KC, etal . Large-scale concatenation cDNA sequencing. . Genome Res. . 7 . 4 . 353–8 . 1997 . 9110174 . 10.1101/gr.7.4.353. 139146 .
• Sparks AB, Hoffman NG, McConnell SJ, etal . Cloning of ligand targets: systematic isolation of SH3 domain-containing proteins. . Nat. Biotechnol. . 14 . 6 . 741–4 . 1998 . 9630982 . 10.1038/nbt0696-741 . 11166026 .
• Nagase T, Ishikawa K, Kikuno R, etal . Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. . DNA Res. . 6 . 5 . 337–45 . 2000 . 10574462 . 10.1093/dnares/6.5.337 . free .
• Pucharcos C, Casas C, Nadal M, etal . The human intersectin genes and their spliced variants are differentially expressed. . Biochim. Biophys. Acta . 1521 . 1–3 . 1–11 . 2001 . 11690630 . 10.1016/s0167-4781(01)00276-7.
• Nakayama M, Kikuno R, Ohara O . Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs. . Genome Res. . 12 . 11 . 1773–84 . 2003 . 12421765 . 10.1101/gr.406902 . 187542 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Chini CC, Chen J . Human claspin is required for replication checkpoint control. . J. Biol. Chem. . 278 . 32 . 30057–62 . 2003 . 12766152 . 10.1074/jbc.M301136200 . free .
• Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs. . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . free .
• Brill LM, Salomon AR, Ficarro SB, etal . Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry. . Anal. Chem. . 76 . 10 . 2763–72 . 2004 . 15144186 . 10.1021/ac035352d .
• Lehner B, Sanderson CM . A protein interaction framework for human mRNA degradation. . Genome Res. . 14 . 7 . 1315–23 . 2004 . 15231747 . 10.1101/gr.2122004 . 442147 .
• Rush J, Moritz A, Lee KA, etal . Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. . Nat. Biotechnol. . 23 . 1 . 94–101 . 2005 . 15592455 . 10.1038/nbt1046 . 7200157 .
• Zhang Y, Wolf-Yadlin A, Ross PL, etal . Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules. . Mol. Cell. Proteomics . 4 . 9 . 1240–50 . 2005 . 15951569 . 10.1074/mcp.M500089-MCP200 . free .
• Nishimura T, Yamaguchi T, Tokunaga A, etal . Role of numb in dendritic spine development with a Cdc42 GEF intersectin and EphB2. . Mol. Biol. Cell . 17 . 3 . 1273–85 . 2006 . 16394100 . 10.1091/mbc.E05-07-0700 . 1382316 .
• Seifert M, Ampofo C, Mehraein Y, etal . Expression analysis of human intersectin 2 gene (ITSN2) minor splice variants showing differential expression in normal human brain. . Oncol. Rep. . 17 . 5 . 1207–11 . 2007 . 17390067 . 10.3892/or.17.5.1207. free .
• Lim CS, Seet BT, Ingham RJ, etal . The K15 protein of Kaposi's sarcoma-associated herpesvirus recruits the endocytic regulator intersectin 2 through a selective SH3 domain interaction. . Biochemistry . 46 . 35 . 9874–85 . 2007 . 17696407 . 10.1021/bi700357s .

Notes and References

1. Pucharcos C, Estivill X, de la Luna S . Intersectin 2, a new multimodular protein involved in clathrin-mediated endocytosis . FEBS Lett . 478 . 1–2 . 43–51 . Aug 2000 . 10922467 . 10.1016/S0014-5793(00)01793-2 . 24683452 .
2. McGavin MK, Badour K, Hardy LA, Kubiseski TJ, Zhang J, Siminovitch KA . The intersectin 2 adaptor links Wiskott Aldrich Syndrome protein (WASp)-mediated actin polymerization to T cell antigen receptor endocytosis . J Exp Med . 194 . 12 . 1777–87 . Dec 2001 . 11748279 . 2193569 . 10.1084/jem.194.12.1777 .
3. Web site: Entrez Gene: ITSN2 intersectin 2.
4. Web site: Entrez Gene: ITSN2 intersectin 2.