ITIH2 explained

Inter-alpha-trypsin inhibitor heavy chain H2 is a protein that in humans is encoded by the ITIH2 gene.^{[1] [2] [3]}

It is known to contain a Gla domain, and thus be dependent for production on post translational modification requiring vitamin K. Its function is also presumably dependent on calcium ions.

See also

• Inter-alpha-trypsin inhibitor
• ITIH1
• ITIH3
• ITIH4

Further reading

• Malki N, Balduyck M, Maes P, etal . The heavy chains of human plasma inter-alpha-trypsin inhibitor: their isolation, their identification by electrophoresis and partial sequencing. Differential reactivity with concanavalin A. . Biol. Chem. Hoppe-Seyler . 373 . 10 . 1009–18 . 1992 . 1384548 . 10.1515/bchm3.1992.373.2.1009.
• Salier JP, Diarra-Mehrpour M, Sesboue R, etal . Isolation and characterization of cDNAs encoding the heavy chain of human inter-alpha-trypsin inhibitor (I alpha TI): unambiguous evidence for multipolypeptide chain structure of I alpha TI. . Proc. Natl. Acad. Sci. U.S.A. . 84 . 23 . 8272–6 . 1988 . 2446322 . 10.1073/pnas.84.23.8272 . 299524 . free .
• Gebhard W, Schreitmüller T, Hochstrasser K, Wachter E . Complementary DNA and derived amino acid sequence of the precursor of one of the three protein components of the inter-alpha-trypsin inhibitor complex. . FEBS Lett. . 229 . 1 . 63–7 . 1988 . 2450046 . 10.1016/0014-5793(88)80798-1 . 6539112 . free .
• Salier JP, Diarra-Mehrpour M, Sesboüé R, etal . Human inter-alpha-trypsin inhibitor. Isolation and characterization of heavy (H) chain cDNA clones coding for a 383 amino-acid sequence of the H chain. . Biol. Chem. Hoppe-Seyler . 369 Suppl . 15–8 . 1989 . 2462430 .
• Diarra-Mehrpour M, Bourguignon J, Sesboüé R, etal . Human plasma inter-alpha-trypsin inhibitor is encoded by four genes on three chromosomes. . Eur. J. Biochem. . 179 . 1 . 147–54 . 1989 . 2465147 . 10.1111/j.1432-1033.1989.tb14532.x .
• Enghild JJ, Thøgersen IB, Pizzo SV, Salvesen G . Analysis of inter-alpha-trypsin inhibitor and a novel trypsin inhibitor, pre-alpha-trypsin inhibitor, from human plasma. Polypeptide chain stoichiometry and assembly by glycan. . J. Biol. Chem. . 264 . 27 . 15975–81 . 1989 . 10.1016/S0021-9258(18)71575-5 . 2476436 . free .
• Schreitmüller T, Hochstrasser K, Reisinger PW, etal . cDNA cloning of human inter-alpha-trypsin inhibitor discloses three different proteins. . Biol. Chem. Hoppe-Seyler . 368 . 8 . 963–70 . 1987 . 3663330 . 10.1515/bchm3.1987.368.2.963.
• Huang L, Yoneda M, Kimata K . A serum-derived hyaluronan-associated protein (SHAP) is the heavy chain of the inter alpha-trypsin inhibitor. . J. Biol. Chem. . 268 . 35 . 26725–30 . 1994 . 10.1016/S0021-9258(19)74373-7 . 7504674 . free .
• Morelle W, Capon C, Balduyck M, etal . Chondroitin sulphate covalently cross-links the three polypeptide chains of inter-alpha-trypsin inhibitor. . Eur. J. Biochem. . 221 . 2 . 881–8 . 1994 . 7513643 . 10.1111/j.1432-1033.1994.tb18803.x .
• Wisniewski HG, Burgess WH, Oppenheim JD, Vilcek J . TSG-6, an arthritis-associated hyaluronan binding protein, forms a stable complex with the serum protein inter-alpha-inhibitor. . Biochemistry . 33 . 23 . 7423–9 . 1994 . 7516184 . 10.1021/bi00189a049 .
• Enghild JJ, Salvesen G, Thøgersen IB, etal . Presence of the protein-glycosaminoglycan-protein covalent cross-link in the inter-alpha-inhibitor-related proteinase inhibitor heavy chain 2/bikunin. . J. Biol. Chem. . 268 . 12 . 8711–6 . 1993 . 10.1016/S0021-9258(18)52933-1 . 7682553 . free .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Mizon C, Balduyck M, Albani D, etal . Development of an enzyme-linked immunosorbent assay for human plasma inter-alpha-trypsin inhibitor (ITI) using specific antibodies against each of the H1 and H2 heavy chains. . J. Immunol. Methods . 190 . 1 . 61–70 . 1996 . 8601712 . 10.1016/0022-1759(95)00257-X .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, etal . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Olsen EH, Rahbek-Nielsen H, Thogersen IB, etal . Posttranslational modifications of human inter-alpha-inhibitor: identification of glycans and disulfide bridges in heavy chains 1 and 2. . Biochemistry . 37 . 1 . 408–16 . 1998 . 9425062 . 10.1021/bi971137d .
• Mizushima S, Nii A, Kato K, Uemura A . Gene expression of the two heavy chains and one light chain forming the inter-alpha-trypsin-inhibitor in human tissues. . Biol. Pharm. Bull. . 21 . 2 . 167–9 . 1998 . 9514613 . 10.1248/bpb.21.167. free .
• Flahaut C, Capon C, Balduyck M, etal . Glycosylation pattern of human inter-alpha-inhibitor heavy chains. . Biochem. J. . 333 . 3. 749–56 . 1998 . 9677337 . 10.1042/bj3330749. 1219641 .
• Hamm A, Veeck J, Bektas N, etal . Frequent expression loss of Inter-alpha-trypsin inhibitor heavy chain (ITIH) genes in multiple human solid tumors: a systematic expression analysis. . BMC Cancer . 8 . 25 . 2008 . 18226209 . 10.1186/1471-2407-8-25 . 25 . 2268946 . free .

Notes and References

1. Salier JP, Simon D, Rouet P, Raguenez G, Muscatelli F, Gebhard W, Guenet JL, Mattei MG . Homologous chromosomal locations of the four genes for inter-alpha-inhibitor and pre-alpha-inhibitor family in human and mouse: assignment of the ancestral gene for the lipocalin superfamily . Genomics . 14 . 1 . 83–8 . Dec 1992 . 1385302 . 10.1016/S0888-7543(05)80287-3 .
2. Jean L, Risler JL, Nagase T, Coulouarn C, Nomura N, Salier JP . The nuclear protein PH5P of the inter-alpha-inhibitor superfamily: a missing link between poly(ADP-ribose)polymerase and the inter-alpha-inhibitor family and a novel actor of DNA repair? . FEBS Lett . 446 . 1 . 6–8 . May 1999 . 10100603 . 10.1016/S0014-5793(99)00173-8 . 84807160 .
3. Web site: Entrez Gene: ITIH2 inter-alpha (globulin) inhibitor H2.