IS061 RNA explained
The ISO61 (IsrA) RNA is a bacterial non-coding RNA that is found between the abgR and ydaL genes in Escherichia coli and Shigella flexneri. It was discovered using a computational screen of the E. coli genome.[1] Subsequent characterisation of ISO61 region has revealed that the reverse strand is actually a CsrA binding ncRNA called McaS and that it has a role in biofilm formation control.[2] Furthermore, it has been shown that McaS(IsrA) exists as ribonucleoprotein particles (sRNPs), which involve a defined set of proteins including Hfq, S1, CsrA, ProQ and PNPase. [3]
See also
Notes and References
- Chen S, Lesnik EA, Hall TA, Sampath R, Griffey RH, Ecker DJ, Blyn LB . A bioinformatics based approach to discover small RNA genes in the Escherichia coli genome . Bio Systems . 65 . 2–3 . 157–177 . 2002 . 12069726 . 10.1016/S0303-2647(02)00013-8 .
- Jørgensen MG, Thomason MK, Havelund J, Valentin-Hansen P, Storz G . Dual function of the McaS small RNA in controlling biofilm formation . Genes & Development . 27 . 10 . 1132–1145 . May 2013 . 23666921 . 3672647 . 10.1101/gad.214734.113 .
- van Nues RW, Castro-Roa D, Yuzenkova Y, Zenkin N . Ribonucleoprotein particles of bacterial small non-coding RNA IsrA (IS61 or McaS) and its interaction with RNA polymerase core may link transcription to mRNA fate . Nucleic Acids Research . 44 . 6 . 2577–2592 . April 2016 . 26609136 . 4824073 . 10.1093/nar/gkv1302 .
