IQSEC1 explained
IQ motif and SEC7 domain-containing protein 1 also known as ARF-GEP100 (ADP-Ribosylation Factor - Guanine nucleotide-Exchange Protein - 100-kDa) is a protein that in humans is encoded by the IQSEC1 gene.[1] [2]
Function
The ARF-GEP100 protein is involved in signal transduction. It is a guanine nucleotide exchange factor that promotes binding of GTP to ADP ribosylation factor protein ARF6 and to a lesser extent ARF1 and ARF5.[2] This activates the ADP-ribosylation activity of the target protein and cause it to modify its substrates. ARF-GEP100, through activation of ARF6, is therefore involved in the control of processes such as endocytosis of plasma membrane proteins, E-cadherin recycling and actin cytoskeleton remodeling.[3] ARF-GEP100 appears particularly important in regulating cell adhesion, with reductions in the level of this protein causing enhanced spreading and attachment of cells.[4]
It is highly expressed in the prefrontal cortex, and throughout the rest of the brain, and is believed to have a role in learning and memory, having been detected as phosphorylated in a phospho screen of the PSD.[5]
Further reading
- Andersson B . A "double adaptor" method for improved shotgun library construction . Anal. Biochem. . 236 . 1 . 107–13 . 1996 . 8619474 . 10.1006/abio.1996.0138 . Wentland MA . Ricafrente JY . Liu . W . Gibbs . RA .
- Yu W . Large-Scale Concatenation cDNA Sequencing . Genome Res. . 7 . 4 . 353–8 . 1997 . 9110174 . 10.1101/gr.7.4.353. 139146 . Andersson B . Worley KC . Muzny . DM . Ding . Y . Liu . W . Ricafrente . JY . Wentland . MA . Lennon . G .
- Nagase T . Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro . DNA Res. . 5 . 5 . 277–86 . 1999 . 9872452 . 10.1093/dnares/5.5.277 . Ishikawa K . Suyama M . Kikuno . R . Miyajima . N . Tanaka . A . Kotani . H . Nomura . N . Ohara . O . free .
- Strausberg RL . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . Feingold EA . Grouse LH . Derge . JG . Klausner . RD . Collins . FS . Wagner . L . Shenmen . CM . Schuler . GD . 2002PNAS...9916899M . free .
- Beausoleil SA . Large-scale characterization of HeLa cell nuclear phosphoproteins . Proc. Natl. Acad. Sci. U.S.A. . 101 . 33 . 12130–5 . 2004 . 15302935 . 10.1073/pnas.0404720101 . 514446 . Jedrychowski M . Schwartz D . Elias . JE . Villén . J . Li . J . Cohn . MA . Cantley . LC . Gygi . SP . 2004PNAS..10112130B . free .
- Gerhard DS . The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . Wagner L . Feingold EA . Shenmen . CM . Grouse . LH . Schuler . G . Klein . SL . Old . S . Rasooly . R .
Notes and References
- Web site: Entrez Gene: IQSEC1 IQ motif and Sec7 domain 1.
- Someya A, Sata M, Takeda K, Pacheco-Rodriguez G, Ferrans VJ, Moss J, Vaughan M . ARF-GEP100, a guanine nucleotide-exchange protein for ADP-ribosylation factor 6 . Proc. Natl. Acad. Sci. U.S.A. . 98 . 5 . 2413–8 . February 2001 . 11226253 . 30152 . 10.1073/pnas.051634798 . 2001PNAS...98.2413S . free .
- Hiroi T, Someya A, Thompson W, Moss J, Vaughan M . GEP100/BRAG2: Activator of ADP-ribosylation factor 6 for regulation of cell adhesion and actin cytoskeleton via E-cadherin and α-catenin . Proc. Natl. Acad. Sci. U.S.A. . 103 . 28 . 10672–7 . 2006 . 16807291 . 10.1073/pnas.0604091103. 1502290 . 2006PNAS..10310672H . free .
- Dunphy JL, Moravec R, Ly K, Lasell TK, Melancon P, Casanova JE . The Arf6 GEF GEP100/BRAG2 regulates cell adhesion by controlling endocytosis of beta1 integrins . Curr. Biol. . 16 . 3 . 315–20 . 2006 . 16461286 . 10.1016/j.cub.2005.12.032 . 3600433 . 2006CBio...16..315D .
- Trinidad JC, Specht CG, Thalhammer A, Schoepfer R, Burlingame AL . Comprehensive identification of phosphorylation sites in postsynaptic density preparations . Mol. Cell. Proteomics . 5 . 5 . 914–22 . May 2006 . 16452087 . 10.1074/mcp.T500041-MCP200 . free .