InaD-like protein explained

InaD-like protein is a protein that in humans is encoded by the PATJ gene.^{[1] [2]}

Function

This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors.^[3]

Interactions

INADL has been shown to interact with MPP5.^[4]

Further reading

• Fanning AS, Anderson JM . PDZ domains: fundamental building blocks in the organization of protein complexes at the plasma membrane . J. Clin. Invest. . 103 . 6 . 767–72 . 1999 . 10079096 . 408156 . 10.1172/JCI6509 .
• Lennon G, Auffray C, Polymeropoulos M, Soares MB . The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression . Genomics . 33 . 1 . 151–2 . 1996 . 8617505 . 10.1006/geno.1996.0177 . free .
• Kurschner C, Mermelstein PG, Holden WT, Surmeier DJ. D. James Surmeier . CIPP, a novel multivalent PDZ domain protein, selectively interacts with Kir4.0 family members, NMDA receptor subunits, neurexins, and neuroligins . Mol. Cell. Neurosci. . 11 . 3 . 161–72 . 1998 . 9647694 . 10.1006/mcne.1998.0679 . 36534759 .
• Vaccaro P, Brannetti B, Montecchi-Palazzi L, Philipp S, Helmer Citterich M, Cesareni G, Dente L . Distinct binding specificity of the multiple PDZ domains of INADL, a human protein with homology to INAD from Drosophila melanogaster . J. Biol. Chem. . 276 . 45 . 42122–30 . 2001 . 11509564 . 10.1074/jbc.M104208200 . free .
• Anzai N, Deval E, Schaefer L, Friend V, Lazdunski M, Lingueglia E . The multivalent PDZ domain-containing protein CIPP is a partner of acid-sensing ion channel 3 in sensory neurons . J. Biol. Chem. . 277 . 19 . 16655–61 . 2002 . 11872753 . 10.1074/jbc.M201087200 . free .
• Roh MH, Makarova O, Liu CJ, Shin K, Lee S, Laurinec S, Goyal M, Wiggins R, Margolis B . The Maguk protein, Pals1, functions as an adapter, linking mammalian homologues of Crumbs and Discs Lost . J. Cell Biol. . 157 . 1 . 161–72 . 2002 . 11927608 . 2173254 . 10.1083/jcb.200109010 .
• Lemmers C, Médina E, Delgrossi MH, Michel D, Arsanto JP, Le Bivic A . hINADl/PATJ, a homolog of discs lost, interacts with crumbs and localizes to tight junctions in human epithelial cells . J. Biol. Chem. . 277 . 28 . 25408–15 . 2002 . 11964389 . 10.1074/jbc.M202196200 . free .
• Roh MH, Liu CJ, Laurinec S, Margolis B . The carboxyl terminus of zona occludens-3 binds and recruits a mammalian homologue of discs lost to tight junctions . J. Biol. Chem. . 277 . 30 . 27501–9 . 2002 . 12021270 . 10.1074/jbc.M201177200 . free .
• Makarova O, Roh MH, Liu CJ, Laurinec S, Margolis B . Mammalian Crumbs3 is a small transmembrane protein linked to protein associated with Lin-7 (Pals1) . Gene . 302 . 1–2 . 21–9 . 2003 . 12527193 . 10.1016/S0378111902010843 .
• Roh MH, Fan S, Liu CJ, Margolis B . The Crumbs3-Pals1 complex participates in the establishment of polarity in mammalian epithelial cells . J. Cell Sci. . 116 . Pt 14 . 2895–906 . 2004 . 12771187 . 10.1242/jcs.00500 . 4482921 .
• Li Y, Karnak D, Demeler B, Margolis B, Lavie A . Structural basis for L27 domain-mediated assembly of signaling and cell polarity complexes . EMBO J. . 23 . 14 . 2723–33 . 2005 . 15241471 . 514954 . 10.1038/sj.emboj.7600294 .
• Michel D, Arsanto JP, Massey-Harroche D, Béclin C, Wijnholds J, Le Bivic A . PATJ connects and stabilizes apical and lateral components of tight junctions in human intestinal cells . J. Cell Sci. . 118 . Pt 17 . 4049–57 . 2005 . 16129888 . 10.1242/jcs.02528 . 19826254 .
• Stiffler MA, Grantcharova VP, Sevecka M, MacBeath G . Uncovering Quantitative Protein Interaction Networks for Mouse PDZ Domains using Protein Microarrays . J. Am. Chem. Soc. . 128 . 17 . 5913–22 . 2007 . 16637659 . 2533859 . 10.1021/ja060943h .
• Wells CD, Fawcett JP, Traweger A, Yamanaka Y, Goudreault M, Elder K, Kulkarni S, Gish G, Virag C, Lim C, Colwill K, Starostine A, Metalnikov P, Pawson T . A Rich1/Amot complex regulates the Cdc42 GTPase and apical-polarity proteins in epithelial cells . Cell . 125 . 3 . 535–48 . 2006 . 16678097 . 10.1016/j.cell.2006.02.045 . 18651810 . free .

Notes and References

1. Philipp S, Flockerzi V . Molecular characterization of a novel human PDZ domain protein with homology to INAD from Drosophila melanogaster . FEBS Lett . 413 . 2 . 243–8 . Sep 1997 . 9280290 . 10.1016/S0014-5793(97)00877-6 . free .
2. Soejima H, Kawamoto S, Akai J, Miyoshi O, Arai Y, Morohka T, Matsuo S, Niikawa N, Kimura A, Okubo K, Mukai T . Isolation of novel heart-specific genes using the BodyMap database . Genomics . 74 . 1 . 115–20 . May 2001 . 11374908 . 10.1006/geno.2001.6527 .
3. Web site: Entrez Gene: INADL InaD-like (Drosophila).
4. Roh MH, Makarova O, Liu CJ, Shin K, Lee S, Laurinec S, Goyal M, Wiggins R, Margolis B . The Maguk protein, Pals1, functions as an adapter, linking mammalian homologues of Crumbs and Discs Lost . J. Cell Biol. . 157 . 1 . 161–72 . Apr 2002 . 11927608 . 2173254 . 10.1083/jcb.200109010 .