IGEPAL CA-630 explained

IGEPAL CA-630 is a nonionic, non-denaturing detergent. Its official IUPAC name is octylphenoxypolyethoxyethanol. IGEPAL is a registered trademark of Rhodia.

IGEPAL CA-630 is sold by Sigma-Aldrich and is claimed to be a "chemically indistinguishable" substitute for Nonidet P-40 (a trademark of Shell Chemical Company) which is no longer manufactured. However, a 2017 publication reported that IGEPAL 630 was ten-fold more potent than Nonidet P-40 in a tubulin polymerisation assay.[1]

All IGEPAL CA surfactants are derived from octylphenol. This serves as the hydrophobic part. Different amounts of ethylene oxide are combined with this part to get a balance of hydrophobic/hydrophilic substances (measured by HLB). This balance has an important impact on wetting detergency, foam, solubility, emulsification. IGEPAL CA-630 has HLB of 13.4, similar to that of Triton X-100 (13.4) and thus belongs to the detergent range (HLB 13-15); this is significantly less than 17.8 of tergitol NP-40 or 16.7 of Polysorbate 20 (also known as Tween 20), which both belong in the solubilizer range (15-18) of HLB.[2] Ca-630 is completely miscible with water.

Human toxicity

CA-630 is not a primary skin irritant nor sensitizer.[3] However, it is a severe eye irritant.[4]

See also

Triton X-100

Notes and References

  1. 10.1139/bcb-2016-0141. Use of substitute Nonidet P-40 nonionic detergents in intracellular tubulin polymerization assays for screening of microtubule targeting agents. Biochemistry and Cell Biology. 95. 3. 379–384. 2017. Sinha. S.. Field. J.J.. Miller. J.H.. 28177759. free.
  2. Web site: The Pharmaceutics and Compounding Laboratory . https://web.archive.org/web/20210513141055/https://pharmlabs.unc.edu/labs/emulsions/hlb.htm . 13 May 2021 . dead.
  3. M.Schick:"Non-ionic Surfactants". p. 929, Marcel Dekker, Inc., N.Y. (1967).
  4. http://www.mpbio.com/detailed_info.php?family_key=02198596{{dead link|date=July 2024|fix-attempted=y}}