Hybrid mass spectrometer explained

A hybrid mass spectrometer is a device for tandem mass spectrometry that consists of a combination of two or more m/z separation devices of different types.

Notation

The different m/z separation elements of a hybrid mass spectrometer can be represented by a shorthand notation. The symbol Q represents a quadrupole mass analyzer, q is a radio frequency collision quadrupole, TOF is a time-of-flight mass spectrometer, B is a magnetic sector and E is an electric sector.

Sector quadrupole

A sector instrument can be combined with a collision quadrupole and quadrupole mass analyzer to form a hybrid instrument.[1] A BEqQ configuration with a magnetic sector (B), electric sector (E), collision quadrupole (q) and m/z selection quadrupole (Q) have been constructed[2] [3] and an instrument with two electric sectors (BEEQ) has been described.[4]

Quadrupole time-of-flight

A triple quadrupole mass spectrometer with the final quadrupole replaced by a time-of-flight device is known as a quadrupole time-of-flight instrument.[5] [6] Such an instrument can be represented as QqTOF.

Ion trap time-of-flight

In an ion trap instrument, ions are trapped in a quadrupole ion trap and then injected into the TOF. The trap can be 3-D[7] or a linear trap.[8]

Linear ion trap and Fourier transform mass analyzers

A linear ion trap combined with a Fourier transform ion cyclotron resonance[9] or Orbitrap[10] [11] [12] mass spectrometer is marketed by Thermo Scientific as the LTQ FT and LTQ Orbitrap, respectively.

Notes and References

  1. Glish . G. . 1982 . A new "hybrid" sector/quadrupole mass spectrometer for mass spectrometry/mass spectrometry . International Journal of Mass Spectrometry and Ion Physics . 41 . 157–177 . 10.1016/0020-7381(82)85032-8 . McLuckey . S . Scott A. McLuckey. Ridley . T . Cooks . R . 3. 1982IJMSI..41..157G .
  2. Schoen . A. . 1985 . A hybrid BEQQ mass spectrometer . International Journal of Mass Spectrometry and Ion Processes . 65 . 125–140 . 10.1016/0168-1176(85)85059-X . Amy . J.W. . Ciupek . J.D. . Cooks . R.G. . Dobberstein . P. . Jung . G. . 1–2 . 1985IJMSI..65..125S .
  3. Harrison . A. . 1986 . A hybrid BEQQ mass spectrometer for studies in gaseous ion chemistry . International Journal of Mass Spectrometry and Ion Processes . 74 . 1 . 13–31 . 10.1016/0168-1176(86)85020-0 . 1986IJMSI..74...13H .
  4. Winger . B. E. . 1992 . Hybrid BEEQ tandem mass spectrometer for the study of ion/surface collision processes . Review of Scientific Instruments . 63 . 5613 . 10.1063/1.1143391 . Laue . H. -J. . Horning . S. R. . Julian . R. K. . Lammert . S. A. . Riederer . D. E. . Cooks . R. G. . 12. 1992RScI...63.5613W .
  5. Shevchenko A, Loboda A, Shevchenko A, Ens W, Standing KG . MALDI quadrupole time-of-flight mass spectrometry: a powerful tool for proteomic research . Anal. Chem. . 72 . 9 . 2132–41 . May 2000 . 10815976 . 10.1021/ac9913659.
  6. Steen H, Küster B, Mann M . Quadrupole time-of-flight versus triple-quadrupole mass spectrometry for the determination of phosphopeptides by precursor ion scanning . J Mass Spectrom . 36 . 7 . 782–90 . July 2001 . 11473401 . 10.1002/jms.174. 2001JMSp...36..782S .
  7. Fountain ST, Lee H, Lubman DM . Ion fragmentation activated by matrix-assisted laser desorption/ionization in an ion-trap/reflectron time-of-flight device . Rapid Commun. Mass Spectrom. . 8 . 5 . 407–16 . May 1994 . 7517726 . 10.1002/rcm.1290080514 . 1994RCMS....8..407F .
  8. Campbell . J. M. . 1998 . A new linear ion trap time-of-flight system with tandem mass spectrometry capabilities . Rapid Communications in Mass Spectrometry . 12 . 1463–1474 . 10.1002/(SICI)1097-0231(19981030)12:20<1463::AID-RCM357>3.0.CO;2-H . Collings . B. A. . Douglas . D. J. . 20.
  9. Syka JE, Marto JA, Bai DL, etal . Novel linear quadrupole ion trap/FT mass spectrometer: performance characterization and use in the comparative analysis of histone H3 post-translational modifications . J. Proteome Res. . 3 . 3 . 621–6 . 2004 . 15253445 . 10.1021/pr0499794.
  10. Makarov A, Denisov E, Kholomeev A, Balschun W, Lange O, Strupat K, Horning S . Performance evaluation of a hybrid linear ion trap/orbitrap mass spectrometer . Anal. Chem. . 78 . 7 . 2113–20 . 2006 . 16579588 . 10.1021/ac0518811.
  11. Olsen JV, de Godoy LM, Li G, etal . Parts per million mass accuracy on an Orbitrap mass spectrometer via lock mass injection into a C-trap . Mol. Cell. Proteomics . 4 . 12 . 2010–21 . December 2005 . 16249172 . 10.1074/mcp.T500030-MCP200 . free .
  12. Yates JR, Cociorva D, Liao L, Zabrouskov V . Performance of a linear ion trap-Orbitrap hybrid for peptide analysis . Anal. Chem. . 78 . 2 . 493–500 . January 2006 . 16408932 . 10.1021/ac0514624.

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