Human lung microvascular endothelial cell explained

Human lung microvascular endothelial cells (HLMVECs) are cells derived from the pulmonary endothelium. HLMVECs are used as a laboratory model system for the study of the function and pathology of the pulmonary endothelium to research conditions such as ARDS^[1] HLMVECs are used due to their simple techniques for isolating them from adult lungs^[2] and their ability to easily proliferate in a laboratory setting. When fully confluent they exhibit a cobblestone phenotype just as they do when lining vessel walls.^[3]

Notes and References

1. Lazo., Colunga-Biancatelli., Solopov., Catravas. . 2023 . An acute respiratory distress syndrome drug development collaboration stimulated by the Virginia Drug Discovery Consortium . SLAS Discovery . 28 . 6 . 249–254 . 10.1016/j.slasd.2023.02.001. 36796645. 9930264.
2. Plebani., D'Alessandro., Lanuti., Simeone., Cinalli., Righi., Palleschi., Mucci., Marchisio., Cappabianca., Camera., Mucilli., Romano. . February 3, 2023 . Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples . JoVE Journal . 192 . 10.3791/64885 .
3. Catravas., Snead., Dimitropoulou., Chang., Lucas., Verin., Black. . 2010 . Harvesting, identification and barrier function of human lung microvascular endothelial cells . Vascular Pharmacology . 52 . 5–6 . 175–81 . 10.1016/j.vph.2009.12.009 . 20060932 . 4374552 .