Heparanase Explained

Heparanase, also known as HPSE, is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate molecules into shorter chain length oligosaccharides.[1] [2]

Synthesis and structure

The protein is originally synthesised in an inactive 65 kDa proheparanase form in the golgi apparatus and transferred to late endosomes/lysosomes for transport to the cell-surface. In the lysosome it is proteolytically processed into its active form. Proteolytic processing results in the production of three products,

The 8 kDa and 50 kDa fragments form a heterodimer and it is this heterodimer that constitutes the active heparanase molecule.[3] The linker protein is so called because prior to its excision it physically links the 8 kDa and 50 kDa proheparanase fragments. Complete excision of the linker peptide appears to be a prerequisite to the complete activation of the heparanase enzyme.

Crystal structures of both proheparanase and mature heparanase are available, showing that the linker peptide forms a large helical domain which blocks heparan sulfate molecules from interacting with heparanase.[4] Removal of the linker reveals an extended cleft on the enzyme surface, which contains the heparanase active site.[5]

Function

Heparanase has endoglycosidase activity and cleaves polymeric heparan sulfate molecules at sites which are internal within the polymeric chain.[6] The enzyme degrades the heparan sulfate scaffold of the basement membrane and extracellular matrix. It is also associated with the inflammatory process, by allowing the extravasation of activated T lymphocytes.[7] In ocular surface physiology this activity functions as an off/on switch for the prosecretory mitogen lacritin. Lacritin binds the cell surface heparan sulfate proteoglycan syndecan-1 only in the presence of active heparanase. Heparanase partially or completely cleaves heparan sulfate to expose a binding site in the N-terminal 50 amino acids of syndecan-1.[8]

Clinical significance

The successful penetration of the endothelial cell layer that lines the interior surface of blood vessels is an important process in the formation of blood borne tumour metastases. Heparan sulfate proteoglycans are major constituents of this layer and it has been shown that increased metastatic potential corresponds with increased heparanase activity for a number of cell lines.[9] [10] Due to the contribution of heparanase activity to metastasis and also to angiogenesis, the inhibition of heparanase activity it is considered to be a potential target for anti-cancer therapies.[11] [12]

Heparanase has been shown to promote arterial thrombosis and stent thrombosis in mouse models due to the cleavage of anti-coagulant heparan sulfate proteoglycans.[13]

Further reading

Notes and References

  1. Vlodavsky I, Friedmann Y, Elkin M, Aingorn H, Atzmon R, Ishai-Michaeli R, Bitan M, Pappo O, Peretz T, Michal I, Spector L, Pecker I . Mammalian heparanase: gene cloning, expression and function in tumor progression and metastasis . Nature Medicine . 5 . 7 . 793–802 . July 1999 . 10395325 . 10.1038/10518 . 38895589 .
  2. Hulett MD, Freeman C, Hamdorf BJ, Baker RT, Harris MJ, Parish CR . Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis . Nature Medicine . 5 . 7 . 803–9 . July 1999 . 10395326 . 10.1038/10525 . 20125382 .
  3. Vlodavsky I, Ilan N, Naggi A, Casu B . Heparanase: structure, biological functions, and inhibition by heparin-derived mimetics of heparan sulfate . Curr. Pharm. Des. . 13 . 20 . 2057–2073 . 2007 . 17627539 . 10.2174/138161207781039742 .
  4. Wu L, Jiang J, Jin Y, Kallemeijn WW, Kuo CL, Artola M, Dai W, van Elk C, van Eijk M, van der Marel GA, ((Codée JDC)), Florea BI, ((Aerts JMFG)), Overkleeft HS, Davies GJ . Activity-based probes for functional interrogation of retaining β-glucuronidases . Nat. Chem. Biol. . 2017 . 28581485 . 10.1038/nchembio.2395 . 13 . 8 . 867–873.
  5. Wu L, Viola CM, Brzozowski AM, Davies GJ . Structural characterization of human heparanase reveals insights into substrate recognition . Nat. Struct. Mol. Biol. . 22 . 12 . 1016–1022 . 2015 . 26575439 . 10.1038/nsmb.3136 . 5008439.
  6. Pikas DS, Li JP, Vlodavsky I, Lindahl U . Substrate specificity of heparanases from human hepatoma and platelets. . J. Biol. Chem. . 273 . 30 . 18770–7 . 1998 . 9668050 . 10.1074/jbc.273.30.18770 . free .
  7. Irony-Tur-Sinai. Michal. Vlodavsky. Israel. Ben-Sasson. Shmuel A. Pinto. Florence. Sicsic. Camille. Brenner. Talma. 2003-01-15. A synthetic heparin-mimicking polyanionic compound inhibits central nervous system inflammation. Journal of the Neurological Sciences. 206. 1. 49–57. 10.1016/S0022-510X(02)00318-0. 12480085. 46339755. 0022-510X.
  8. Ma P, Beck SL, Raab RW, McKown RL, Coffman GL, Utani A, Chirico WJ, Rapraeger AC, Laurie GW . Heparanase deglycanation of syndecan-1 is required for binding of the epithelial-restricted prosecretory mitogen lacritin . The Journal of Cell Biology . 174 . 7 . 1097–106 . September 2006 . 16982797 . 1666580 . 10.1083/jcb.200511134 .
  9. Nakajima M, Irimura T, Nicolson GL . Heparanases and tumor metastasis . J. Cell. Biochem. . 36 . 2 . 157–167 . 1988 . 3281960 . 10.1002/jcb.240360207 . 9743270 .
  10. Vlodavsky I, Goldshmidt O, Zcharia E, Atzmon R, Rangini-Guatta Z, Elkin M, Peretz T, Friedmann Y . Mammalian heparanase: involvement in cancer metastasis, angiogenesis and normal development . Seminars in Cancer Biology . 12 . 2 . 121–9 . 2003 . 12027584 . 10.1006/scbi.2001.0420 .
  11. Yang. Jian-min. Wang. Hui-ju. Du. Ling. Han. Xiao-mei. Ye. Zai-yuan. Fang. Yong. Tao. Hou-quan. Zhao. Zhong-sheng. Zhou. Yong-lie. 2009-01-25. Screening and identification of novel B cell epitopes in human heparanase and their anti-invasion property for hepatocellular carcinoma. Cancer Immunology, Immunotherapy. en. 58. 9. 1387–1396. 10.1007/s00262-008-0651-x. 19169879. 19074169. 0340-7004. 11030199.
  12. Overexpression of heparanase multiple antigenic peptide 2 is associated with poor prognosis in gastric cancer: Potential for therapy. Oncology Letters. 4. 1. 178–182. 2016-03-29. 10.3892/ol.2012.703. 22807984. 2012. Zhang. JUN. Yang. Jianmin. Han. Xiaomei. Zhao. Zhongsheng. Du. Ling. Yu. Tong. Wang. Huiju. 3398369.
  13. Baker AB, Gibson WJ, Kolachalama VB, Golomb M, Indolfi L, Spruell C, Zcharia E, Vlodavsky I, Edelman ER . Heparanase regulates thrombosis in vascular injury and stent-induced flow disturbance. . J Am Coll Cardiol . 59 . 17 . 1551–60 . 2012 . 22516446 . 4191917 . 10.1016/j.jacc.2011.11.057 .