| Holy Cross High School | |
| Motto: | Real Life, Real Learning |
| Streetaddress: | 5144 Dixie Highway |
| City: | Louisville |
| State: | Kentucky |
| Zipcode: | 40216 |
| Country: | United States |
| Coordinates: | 38.1658°N -85.83°W |
| Religion: | Roman Catholic |
| Oversight: | Archdiocese of Louisville |
| Superintendent: | Leisa Schulz |
| President: | Danielle Wiegandt |
| Principal: | Jennifer Barz |
| Chaplain: | Fr. Bill Burks |
| Faculty: | 30 |
| Gender: | Coed |
| Type: | Private, college-preparatory school |
| Grades: | 9–12 |
| Campus: | Suburban |
| Campus Size: | 23acres |
| Athletics: | KHSAA |
| Slogan: | Real Life, Real Learning |
| Nickname: | Cougars |
| Colors: | Maroon, grey and white |
| Status: | Open |
| Enrollment: | 262[1] |
| Enrollment As Of: | 2015-16 |
| Free Label1: | Preceded by |
| Free Text1: | Bishop David & Angela Merici |
Holy Cross High School is a coeducational Catholic high school located in Louisville, Kentucky, United States.