Hesperonatalius harrietae is a species of mygalomorph spider in the Anamidae family. It is endemic to Australia. It was described in 2017 by Mark Castalanelli, Joel Huey, Mia Hillyer and Mark Harvey.[1]
The species occurs in Western Australia in the Geraldton Sandplains bioregion. The type locality is Oakajee, 25 km north of Geraldton.[1]