Heller's test explained

Heller's test is a chemical test that shows that strong acids cause the denaturation of precipitated proteins. Concentrated nitric acid is added to a protein solution from the side of the test tube to form two layers. A white ring appears between the two layers if the test is positive.^[1] Heller's test is commonly used to test for the presence of proteins in urine.^[2] This test was discovered by the Austrian Chemist, Johann Florian Heller (1813-1871).

Notes and References

1. Book: Dandekar. Practicals And Viva In Medical Biochemistry. 1 January 2004. Elsevier India. 978-81-8147-025-6. 26.
   - Book: Nigam. Lab Manual in Biochemistry: Immunology and Biotechnology. 1 April 2007. Tata McGraw-Hill Education. 978-0-07-061767-4. 152.
   - Book: Chawla. Practical Clinical Biochemistry: Methods and Interpretations. 1 January 2003. Jaypee Brothers Publishers. 978-81-8061-108-7. 59.
   - Book: A.C. Croftan. Clinical Urinology. Рипол Классик. 978-1-275-01265-3. 115.
2. Book: Elizabeth A. Martin. Concise Colour Medical Dictionary. 25 February 2010. Oxford University Press. 978-0-19-955715-8. 335.