Immunoglobulin heavy chain explained

The immunoglobulin heavy chain (IgH) is the large polypeptide subunit of an antibody (immunoglobulin). In human genome, the IgH gene loci are on chromosome 14.

A typical antibody is composed of two immunoglobulin (Ig) heavy chains and two Ig light chains. Several different types of heavy chain exist that define the class or isotype of an antibody. These heavy chain types vary between different animals. All heavy chains contain a series of immunoglobulin domains, usually with one variable domain (V_H) that is important for binding antigen and several constant domains (C_H1, C_H2, etc.). Production of a viable heavy chain is a key step in B cell maturation. If the heavy chain is able to bind to a surrogate light chain and move to the plasma membrane, then the developing B cell can begin producing its light chain.^[1]

The heavy chain does not always have to bind to a light chain. Pre-B lymphocytes can synthesize heavy chain in the absence of light chain, which then can allow the heavy chain to bind to a heavy-chain binding protein.^[2]

In mammals

Classes

There are five types of mammalian immunoglobulin heavy chain: γ, δ, α, μ and ε.^[3] They define classes of immunoglobulins: IgG, IgD, IgA, IgM and IgE, respectively.

• Heavy chains α and γ have approximately 450 amino acids.
• Heavy chains μ and ε have approximately 550 amino acids.^[3]

Regions

Each heavy chain has two regions:

• a constant region (which is the same for all immunoglobulins of the same class but differs between classes).
  • Heavy chains γ, α and δ have a constant region composed of three tandem (in a line next to each other) immunoglobulin domains but also have a hinge region for added flexibility.^[4]
  • Heavy chains μ and ε have a constant region composed of four domains.^[3]
• a variable region that differs between different B cells, but is the same for all immunoglobulins produced by the same B cell or B cell clone. The variable domain of any heavy chain is composed of a single immunoglobulin domain. These domains are about 110 amino acids long.^[5]

Cows

Cows, specifically Bos taurus, show a variation on the general mammalian theme in which the heavy chain CDR H3 region has adapted to produce a divergent repertoire of antibodies which present a "stalk and knob" antigen interaction surface instead of the more familiar bivalent tip surface.^[6] The bovine CDR is unusually long and contains unique sequence attributes which support the production of paired cysteine residues during somatic hypermutation.^[6] Thus, where in humans the somatic hypermutation step targets the V(D)J recombination process, the target in cows is on the creation of diverse disulfide bonds and the generation of unique sets of loops which interact with antigen.^[6] A speculated evolutionary driver for this variation is the presence of a vastly more diverse microbial environment in the digestive system of the cow as a consequence of their being ruminants.^[6]

In fish

Jawed fish appear to be the most primitive animals that are able to make antibodies like those described for mammals.^[7] However, fish do not have the same repertoire of antibodies that mammals possess.^[8] Three distinct Ig heavy chains have so far been identified in bony fish.

• The first identified was the μ (or mu) heavy chain that is present in all jawed fish and is the heavy chain for what is thought to be the primordial immunoglobulin. The resulting antibody, IgM, is secreted as a tetramer in teleost fish instead of the typical pentamer found in mammals and sharks.
• The heavy chain (δ) for IgD was identified initially from the channel catfish and Atlantic salmon and is now well documented for many teleost fish.^[9]
• The third teleost Ig heavy chain gene was identified very recently and does not resemble any of the heavy chains so far described for mammals. This heavy chain, identified in both rainbow trout (τ)^[10] and zebrafish (ζ),^[11] could potentially form a distinct antibody isotype (IgT or IgZ) that may precede IgM in evolutionary terms.

Similar to the situation observed for bony fish, three distinct Ig heavy chain isotypes have been identified in cartilaginous fish. With the exception of μ, these Ig heavy chain isotypes appear to be unique to cartilaginous fish. The resulting antibodies are designated IgW (also called IgX or IgNARC) and IgNAR (immunoglobulin new antigen receptor).^{[12] [13]} The latter type is a heavy-chain antibody, an antibody lacking light chains, and can be used to produce single-domain antibodies, which are essentially the variable domain (V_NAR) of an IgNAR.^{[14] [15] [16]} Shark single domain antibodies (V_NARs) to tumor or viral antigens can be isolated from a large naïve nurse shark V_NAR library using phage display technology.^[17]

IgW has now also been found in the group of lobe finned fishes including the coelacanth and lungfish. The IgW1 and IgW2 in coelacanth has a usual (VD)n-Jn-C structure as well as having a large number of constant domains.^{[18] [19]}

In amphibians

Frogs can synthesize IgX and IgY. ^[20]

See also

• Heavy-chain antibody

External links

• • Educational Resource for Heavy Chain Analysis

Notes and References

1. Mårtensson. I-L. Ceredig. R. 2017-01-23. Role of the surrogate light chain and the pre-B-cell receptor in mouse B-cell development. Immunology. 101. 4. 435–441. 10.1046/j.1365-2567.2000.00151.x. 0019-2805. 2327112. 11122446.
2. 10.1038/306387a0. 6417546. Immunoglobulin heavy chain binding protein. Nature. 306. 5941. 387–9. 1983. Haas. Ingrid G.. Wabl. Matthias. 1983Natur.306..387H. 4247626.
3. Book: Janeway CA, Jr. . Immunobiology . 5th . Garland Publishing . 2001 . (electronic full text via NCBI Bookshelf) . 0-8153-3642-X . etal . registration . Charles Janeway .
4. 10.1038/nri1266. 15040582. Human antibody–Fc receptor interactions illuminated by crystal structures. Nature Reviews Immunology. 4. 2. 89–99. 2004. Woof. Jenny M.. Burton. Dennis R.. 30584218.
5. Web site: The Biology Project . Antibody Structure . The University of Arizona . May 27, 2020.
6. 10.1016/j.cell.2013.04.049. 23746848. Reshaping Antibody Diversity. Cell. 153. 6. 1379–93. 2013. Wang. Feng. Ekiert. Damian C.. Ahmad. Insha. Yu. Wenli. Zhang. Yong. Bazirgan. Omar. Torkamani. Ali. Raudsepp. Terje. Mwangi. Waithaka. Criscitiello. Michael F.. Wilson. Ian A.. Schultz. Peter G.. Smider. Vaughn V.. 4007204.
7. http://www.medicine.uiowa.edu/cigw/fish.htm Fish heavy chain and light chain genes
8. 10.1016/j.dci.2005.06.016. 16153707. Channel catfish immunoglobulins: Repertoire and expression. Developmental & Comparative Immunology. 30. 77–92. 2006. Bengtén. Eva. Clem. L. William. Miller. Norman W.. Warr. Gregory W.. Wilson. Melanie. 1–2.
9. 10.1016/j.dci.2005.06.007. 16084588. Antibody repertoire development in teleosts—a review with emphasis on salmonids and Gadus morhua L. Developmental & Comparative Immunology. 30. 57–76. 2006. Solem. Stein Tore. Stenvik. Jørgen. 1–2.
10. 2005PNAS..102.6919H . 3375456 . 1100771. 2005 . Hansen . J. D. . Discovery of a unique Ig heavy-chain isotype (IgT) in rainbow trout: Implications for a distinctive B cell developmental pathway in teleost fish . Proceedings of the National Academy of Sciences . 102 . 19 . 6919–6924 . Landis . E. D. . Phillips . R. B. . 10.1073/pnas.0500027102 . 15863615. free .
11. 10.1038/ni1166. 15685175. The immunoglobulin heavy-chain locus in zebrafish: Identification and expression of a previously unknown isotype, immunoglobulin Z. Nature Immunology. 6. 3. 295–302. 2005. Danilova. Nadia. Bussmann. Jeroen. Jekosch. Kerstin. Steiner. Lisa A. 5543330.
12. 10.1016/j.dci.2005.06.022. 16146649. Antibody repertoire development in cartilaginous fish. Developmental & Comparative Immunology. 30. 43–56. 2006. Dooley. H.. Flajnik. M.F.. 1–2.
13. 10.1016/j.jim.2006.07.019. 16962608. Dimerisation strategies for shark IgNAR single domain antibody fragments. Journal of Immunological Methods. 315. 1–2. 171–84. 2006. Simmons. David P.. Abregu. Fiona A.. Krishnan. Usha V.. Proll. David F.. Streltsov. Victor A.. Doughty. Larissa. Hattarki. Meghan K.. Nuttall. Stewart D..
14. 10.1007/s00430-009-0116-7. 19529959. Single domain antibodies: Promising experimental and therapeutic tools in infection and immunity. Medical Microbiology and Immunology. 198. 3. 157–74. 2009. Wesolowski. Janusz. Alzogaray. Vanina. Reyelt. Jan. Unger. Mandy. Juarez. Karla. Urrutia. Mariela. Cauerhff. Ana. Danquah. Welbeck. Rissiek. Björn. Scheuplein. Felix. Schwarz. Nicole. Adriouch. Sahil. Boyer. Olivier. Seman. Michel. Licea. Alexei. Serreze. David V.. Goldbaum. Fernando A.. Haag. Friedrich. Koch-Nolte. Friedrich. 2714450.
15. Feng. Mingqian. Bian. Hejiao. Wu. Xiaolin. Fu. Tianyun. Fu. Ying. Hong. Jessica. Fleming. Bryan D.. Flajnik. Martin F.. Ho. Mitchell. January 2019. Construction and next-generation sequencing analysis of a large phage-displayed VNAR single-domain antibody library from six naïve nurse sharks. Antibody Therapeutics. 2. 1. 1–11. 10.1093/abt/tby011. 2516-4236. 6312525. 30627698.
16. English. Hejiao. Hong. Jessica. Ho. Mitchell. Ancient species offers contemporary therapeutics: an update on shark VNAR single domain antibody sequences, phage libraries and potential clinical applications. Antibody Therapeutics. 3. 1–9. en. 10.1093/abt/tbaa001. 2020. 1. 32118195. 7034638. free.
17. Li. Dan. English. Hejiao. Hong. Jessica. Liang. Tianyuzhou. Merlino. Glenn. Day. Chi-Ping. Ho. Mitchell. 2021-07-21. A novel PD-L1-targeted shark VNAR single domain-based CAR-T strategy for treating breast cancer and liver cancer. en. 2021.07.20.453144. 10.1101/2021.07.20.453144. 236203365 .
18. Zhang . Tianyi . Tacchi . Luca . Wei . Zhiguo . Zhao . Yaofeng . Salinas . Irene . Intraclass diversification of immunoglobulin heavy chain genes in the African lungfish . Immunogenetics . 66 . 5 . 335–51 . 2014 . 24676685 . 4348116 . 10.1007/s00251-014-0769-2 .
19. Ota . T. . Rast . J. P. . Litman . G. W. . Amemiya . C. T. . Lineage-restricted retention of a primitive immunoglobulin heavy chain isotype within the Dipnoi reveals an evolutionary paradox . Proceedings of the National Academy of Sciences . 100 . 5 . 2501–6 . 2003 . 12606718 . 151370 . 10.1073/pnas.0538029100 . 2003PNAS..100.2501O . free .
20. 10.1016/j.vetimm.2011.10.019. 22100190. 3273591. Oral immunization of the African clawed frog (Xenopus laevis) upregulates the mucosal immunoglobulin IgX. Veterinary Immunology and Immunopathology. 145. 1–2. 493–8. 2012. Du. Christina C.. Mashoof. Sara M.. Criscitiello. Michael F..