AGFG1 explained

Arf-GAP domain and FG repeat-containing protein 1 is a protein that in humans is encoded by the AGFG1 gene.^{[1] [2]}

Function

The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. This encoded protein binds the Rev activation domain when Rev is assembled onto its RNA target and can significantly enhance Rev activity when overexpressed. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.^[2]

Interactions

AGFG1 has been shown to interact with EPS15L1^[3] and EPS15.^[3]

Further reading

• Li L, Li HS, Pauza CD, Bukrinsky M, Zhao RY . Roles of HIV-1 auxiliary proteins in viral pathogenesis and host-pathogen interactions . Cell Res. . 15 . 11–12 . 923–34 . 2006 . 16354571 . 10.1038/sj.cr.7290370 . free .
• Bogerd HP, Fridell RA, Madore S, Cullen BR . Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins . Cell . 82 . 3 . 485–94 . 1995 . 7634337 . 10.1016/0092-8674(95)90437-9 . 18962536 . free .
• Fridell RA, Bogerd HP, Cullen BR . Nuclear export of late HIV-1 mRNAs occurs via a cellular protein export pathway . Proc. Natl. Acad. Sci. U.S.A. . 93 . 9 . 4421–4 . 1996 . 8633082 . 39553 . 10.1073/pnas.93.9.4421 . 1996PNAS...93.4421F . free .
• Fritz CC, Green MR . HIV Rev uses a conserved cellular protein export pathway for the nucleocytoplasmic transport of viral RNAs . Curr. Biol. . 6 . 7 . 848–54 . 1997 . 8805303 . 10.1016/S0960-9822(02)00608-5 . 16705807 . free .
• Stutz F, Izaurralde E, Mattaj IW, Rosbash M . A role for nucleoporin FG repeat domains in export of human immunodeficiency virus type 1 Rev protein and RNA from the nucleus . Mol. Cell. Biol. . 16 . 12 . 7144–50 . 1997 . 8943370 . 231718 . 10.1128/mcb.16.12.7144.
• Jones T, Sheer D, Bevec D, Kappel B, Hauber J, Steinkasserer A . The human HIV-1 Rev binding-protein hRIP/Rab (HRB) maps to chromosome 2q36 . Genomics . 40 . 1 . 198–9 . 1997 . 9070945 . 10.1006/geno.1996.4457 .
• Salcini AE, Confalonieri S, Doria M, Santolini E, Tassi E, Minenkova O, Cesareni G, Pelicci PG, Di Fiore PP . Binding specificity and in vivo targets of the EH domain, a novel protein-protein interaction module . Genes Dev. . 11 . 17 . 2239–49 . 1997 . 9303539 . 275390 . 10.1101/gad.11.17.2239 .
• Neville M, Stutz F, Lee L, Davis LI, Rosbash M . The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export . Curr. Biol. . 7 . 10 . 767–75 . 1998 . 9368759 . 10.1016/S0960-9822(06)00335-6 . 6827586 . free .
• Güre AO, Altorki NK, Stockert E, Scanlan MJ, Old LJ, Chen YT . Human lung cancer antigens recognized by autologous antibodies: definition of a novel cDNA derived from the tumor suppressor gene locus on chromosome 3p21.3 . Cancer Res. . 58 . 5 . 1034–41 . 1998 . 9500467 .
• Farjot G, Sergeant A, Mikaélian I . A new nucleoporin-like protein interacts with both HIV-1 Rev nuclear export signal and CRM-1 . J. Biol. Chem. . 274 . 24 . 17309–17 . 1999 . 10358091 . 10.1074/jbc.274.24.17309 . free .
• Doria M, Salcini AE, Colombo E, Parslow TG, Pelicci PG, Di Fiore PP . The eps15 homology (EH) domain-based interaction between eps15 and hrb connects the molecular machinery of endocytosis to that of nucleocytosolic transport . J. Cell Biol. . 147 . 7 . 1379–84 . 2000 . 10613896 . 2174238 . 10.1083/jcb.147.7.1379 .
• Dias Neto E, Correa RG, Verjovski-Almeida S, Briones MR, Nagai MA, da Silva W, Zago MA, Bordin S, Costa FF, Goldman GH, Carvalho AF, Matsukuma A, Baia GS, Simpson DH, Brunstein A, de Oliveira PS, Bucher P, Jongeneel CV, O'Hare MJ, Soares F, Brentani RR, Reis LF, de Souza SJ, Simpson AJ . Shotgun sequencing of the human transcriptome with ORF expressed sequence tags . Proc. Natl. Acad. Sci. U.S.A. . 97 . 7 . 3491–6 . 2000 . 10737800 . 16267 . 10.1073/pnas.97.7.3491 . 2000PNAS...97.3491D . free .
• Yang J, Bogerd HP, Wang PJ, Page DC, Cullen BR . Two closely related human nuclear export factors utilize entirely distinct export pathways . Mol. Cell . 8 . 2 . 397–406 . 2001 . 11545741 . 10.1016/S1097-2765(01)00303-3 . free .
• Chen D, Li X, Zhai Z, Shu HB . A novel zinc finger protein interacts with receptor-interacting protein (RIP) and inhibits tumor necrosis factor (TNF)- and IL1-induced NF-kappa B activation . J. Biol. Chem. . 277 . 18 . 15985–91 . 2002 . 11854271 . 10.1074/jbc.M108675200 . free .
• Scherl A, Couté Y, Déon C, Callé A, Kindbeiter K, Sanchez JC, Greco A, Hochstrasser D, Diaz JJ . Functional proteomic analysis of human nucleolus . Mol. Biol. Cell . 13 . 11 . 4100–9 . 2003 . 12429849 . 133617 . 10.1091/mbc.E02-05-0271 .
• Kiss A, Li L, Gettemeier T, Venkatesh LK . Functional analysis of the interaction of the human immunodeficiency virus type 1 Rev nuclear export signal with its cofactors . Virology . 314 . 2 . 591–600 . 2003 . 14554087 . 10.1016/S0042-6822(03)00531-2 .
• Sánchez-Velar N, Udofia EB, Yu Z, Zapp ML . hRIP, a cellular cofactor for Rev function, promotes release of HIV RNAs from the perinuclear region . Genes Dev. . 18 . 1 . 23–34 . 2004 . 14701878 . 314270 . 10.1101/gad.1149704 .

Notes and References

1. Fritz CC, Zapp ML, Green MR . A human nucleoporin-like protein that specifically interacts with HIV Rev . Nature . 376 . 6540 . 530–3 . Sep 1995 . 7637788 . 10.1038/376530a0 . 1995Natur.376..530F . 4252707 .
2. Web site: Entrez Gene: HRB HIV-1 Rev binding protein.
3. Doria M, Salcini AE, Colombo E, Parslow TG, Pelicci PG, Di Fiore PP . The eps15 homology (EH) domain-based interaction between eps15 and hrb connects the molecular machinery of endocytosis to that of nucleocytosolic transport . J. Cell Biol. . 147 . 7 . 1379–84 . Dec 1999 . 10613896 . 2174238 . 10.1083/jcb.147.7.1379 .