Unbeaten spent much of her career operating in the Mediterranean, where she sank the Italian sailing vessel V 51 / Alfa, the Vichy-French merchant PLM 20, the and the German submarine . She also claimed to have sunk two sailing vessels with gunfire on 15 July 1941 at Marsa Zuag roads, Libya, but Italian sources only confirm damage to one fishing vessel.
Unbeaten also lightly damaged the Italian merchant Vettor Pisani on 16 March 1942.[1] She also unsuccessfully attacked the Italian merchant Silvio Scaroni, the Italian troop transport Esperia and a large Italian troop transport, thought to be either Oceania or Neptunia.[2]
After a refit in Chatham, and subsequent workup, Unbeaten was attached to the Third Submarine Flotilla in Scotland. Having sailed from Holy Loch on her last patrol, Unbeaten completed Operation Bluestone, landing an agent in Spain near Bayona. She then completed her patrol in the Bay of Biscay and was returning to the United Kingdom when she went missing. It is believed that she was probably attacked and sunk in error by a Royal Air Force Wellington of No. 172 Squadron, Coastal Command in the Bay of Biscay on 11 November 1942. She was lost with all hands.[3]