| Image Coa: | Wappen Groß Twülpstedt.png |
| Coordinates: | 52.3667°N 65°W |
| Image Plan: | Groß Twülpstedt in HE.svg |
| State: | Niedersachsen |
| District: | Helmstedt |
| Samtgemeinde: | Velpke |
| Elevation: | 103 |
| Area: | 36.43 |
| Postal Code: | 38464 |
| Area Code: | 05364 |
| Licence: | HE |
| Gemeindeschlüssel: | 03 1 54 009 |
| Divisions: | 7 |
| Mayor: | Hans-Ulrich Rothe |
| Party: | SPD |
Groß Twülpstedt is a municipality in the district of Helmstedt, in Lower Saxony, Germany. The Municipality Groß Twülpstedt includes the villages of Groß Sisbeck, Groß Twülpstedt, Klein Sisbeck, Klein Twülpstedt, Papenrode, Rümmer and Volkmarsdorf.