Glycyl endopeptidase explained

Glycyl endopeptidase
Ec Number:	3.4.22.25
Cas Number:	149719-24-4

Glycyl endopeptidase (papaya peptidase B, papaya proteinase IV, glycine-specific proteinase, chymopapain, Papaya proteinase 4, PPIV, chymopapain M) is an enzyme.^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

Preferential cleavage: Gly-, in proteins and small molecule substrates

This enzyme is isolated from the papaya plant, Carica papaya.

Notes and References

1. Polgár L . Isolation of highly active papaya peptidases A and B from commercial chymopapain . Biochimica et Biophysica Acta (BBA) - Enzymology . 658 . 2 . 262–9 . April 1981 . 7018581 . 10.1016/0005-2744(81)90296-5 .
2. Buttle DJ, Kembhavi AA, Sharp SL, Shute RE, Rich DH, Barrett AJ . Affinity purification of the novel cysteine proteinase papaya proteinase IV, and papain from papaya latex . The Biochemical Journal . 261 . 2 . 469–76 . July 1989 . 10.1042/bj2610469 . 2505761 . 1138849 .
3. Ritonja A, Buttle DJ, Rawlings ND, Turk V, Barrett AJ . Papaya proteinase IV amino acid sequence . FEBS Letters . 258 . 1 . 109–12 . November 1989 . 2591528 . 10.1016/0014-5793(89)81627-8 . free .
4. Buttle DJ, Ritonja A, Pearl LH, Turk V, Barrett AJ . Selective cleavage of glycyl bonds by papaya proteinase IV . FEBS Letters . 260 . 2 . 195–7 . January 1990 . 2404797 . 10.1016/0014-5793(90)80101-n . free .
5. Buttle DJ, Ritonja A, Dando PM, Abrahamson M, Shaw EN, Wikstrom P, Turk V, Barrett AJ . Interactions of papaya proteinase IV with inhibitors . FEBS Letters . 262 . 1 . 58–60 . March 1990 . 1690669 . 10.1016/0014-5793(90)80153-a . free .