Glycoside hydrolase family 42 explained
| Symbol: | Glyco_hydro_42 |
| Beta-galactosidase |
| Pfam: | PF02449 |
| Pfam Clan: | CL0058 |
| Interpro: | IPR013529 |
| Scop: | 1kwg |
| Cazy: | GH42 |
| Symbol: | Glyco_hydro_42M |
| Beta-galactosidase trimerisation domain |
| Pfam: | PF08532 |
| Pfam Clan: | CL0014 |
| Interpro: | IPR013738 |
| Scop: | 1kwg |
| Symbol: | Glyco_hydro_42C |
| Beta-galactosidase C-terminal domain |
| Pfam: | PF08533 |
| Pfam Clan: | CL0369 |
| Interpro: | IPR013739 |
| Scop: | 1kwg |
In molecular biology, glycoside hydrolase family 42 is a family of glycoside hydrolases.
Glycoside hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1] [2] [3] This classification is available on the CAZy web site,[4] [5] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[6] [7]
The glycosyl hydrolase 42 family CAZY GH_42 comprises beta-galactosidase enzymes . These enzyme catalyse the hydrolysis of terminal, non-reducing terminal beta-D-galactoside residues. The middle domain of these three-domain enzymes is involved in trimerisation.[8]
Notes and References
- Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G . Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases . Proceedings of the National Academy of Sciences of the United States of America . 92 . 15 . 7090–4 . July 1995 . 7624375 . 41477 . 10.1073/pnas.92.15.7090 . 1995PNAS...92.7090H . free .
- Davies G, Henrissat B . Structures and mechanisms of glycosyl hydrolases . Structure . 3 . 9 . 853–9 . September 1995 . 8535779 . 10.1016/S0969-2126(01)00220-9 . free .
- Henrissat B, Bairoch A . Updating the sequence-based classification of glycosyl hydrolases . The Biochemical Journal . 316 . Pt 2 . 695–6 . June 1996 . 8687420 . 1217404 . 10.1042/bj3160695.
- Web site: Home. CAZy.org. en. 2018-03-06.
- Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B . The carbohydrate-active enzymes database (CAZy) in 2013 . Nucleic Acids Research . 42 . Database issue . D490-5 . January 2014 . 24270786 . 3965031 . 10.1093/nar/gkt1178 .
- Web site: Glycoside Hydrolase Family 42. CAZypedia.org. en. 2018-03-06.
- Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes . Glycobiology . 28 . 1 . 3–8 . December 2018 . 29040563 . 10.1093/glycob/cwx089 . CAZypedia Consortium . free .
- Shimizu T, Kobayashi T, Ba-Thein W, Ohtani K, Hayashi H . Sequence analysis of flanking regions of the pfoA gene of Clostridium perfringens: beta-galactosidase gene (pbg) is located in the 3'-flanking region . Microbiology and Immunology . 39 . 9 . 677–86 . 1995 . 8577281 . 10.1111/j.1348-0421.1995.tb03256.x . 2650178 . free .
