Glycoside hydrolase family 4 explained
| Symbol: | Glyco_hydro_4 |
| Family 4 glycosyl hydrolase |
| Pfam: | PF02056 |
| Pfam Clan: | CL0063 |
| Prosite: | PDOC01027 |
| Scop: | 1obb |
| Cazy: | GH4 |
| Interpro: | IPR001088 |
| Symbol: | Glyco_hydro_4C |
| Family 4 glycosyl hydrolase C-terminal domain |
| Pfam: | PF11975 |
| Pfam Clan: | CL0341 |
| Prosite: | PDOC01027 |
| Scop: | 1obb |
| Cazy: | GH4 |
In molecular biology, glycoside hydrolase family 4 is a family of glycoside hydrolases, which are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1] [2] [3] This classification is available on the CAZy web site,[4] [5] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[6] [7]
Glycoside hydrolase family 4 CAZY GH_4 comprises enzymes with several known activities; 6-phospho-beta-glucosidase ; 6-phospho-alpha-glucosidase ; alpha-galactosidase ; alpha-D-glucuronidase (EC 3.2.1.139).[8] 6-phospho-alpha-glucosidase requires both NAD(H) and divalent metal (Mn2+, Fe2+, Co2+, or Ni2+) for activity.[9]
External references
GH4 in CAZypedia
Notes and References
- Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G . Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases . Proceedings of the National Academy of Sciences of the United States of America . 92 . 15 . 7090–4 . July 1995 . 7624375 . 41477 . 10.1073/pnas.92.15.7090 . 1995PNAS...92.7090H . free .
- Davies G, Henrissat B . Structures and mechanisms of glycosyl hydrolases . Structure . 3 . 9 . 853–9 . September 1995 . 8535779 . 10.1016/S0969-2126(01)00220-9 . free .
- Henrissat B, Bairoch A . Updating the sequence-based classification of glycosyl hydrolases . The Biochemical Journal . 316 . Pt 2 . 695–6 . June 1996 . 8687420 . 1217404 . 10.1042/bj3160695.
- Web site: Home. CAZy.org. en. 2018-03-06.
- Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B . The carbohydrate-active enzymes database (CAZy) in 2013 . Nucleic Acids Research . 42 . Database issue . D490-5 . January 2014 . 24270786 . 3965031 . 10.1093/nar/gkt1178 .
- Web site: Glycoside Hydrolase Family 4. CAZypedia.org. en. 2018-03-06.
- Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes . Glycobiology . 28 . 1 . 3–8 . December 2018 . 29040563 . 10.1093/glycob/cwx089 . CAZypedia Consortium . free .
- Mohapatra . Samar Ballabha . Manoj . Narayanan . 2021-02-26 . Structural basis of catalysis and substrate recognition by the NAD(H)-dependent α-d-glucuronidase from the glycoside hydrolase family 4 . Biochemical Journal . en . 478 . 4 . 943–959 . 10.1042/BCJ20200824 . 33565573 . 0264-6021. free .
- Thompson J, Pikis A, Ruvinov SB, Henrissat B, Yamamoto H, Sekiguchi J . The gene glvA of Bacillus subtilis 168 encodes a metal-requiring, NAD(H)-dependent 6-phospho-alpha-glucosidase. Assignment to family 4 of the glycosylhydrolase superfamily . The Journal of Biological Chemistry . 273 . 42 . 27347–56 . October 1998 . 9765262 . 10.1074/jbc.273.42.27347 . free .
