| Dame Gladys Taylor | |
| Death Place: | Westminster Hospital, London |
| Allegiance: | United Kingdom |
| Branch: | Royal Air Force |
| Rank: | Matron-in-Chief |
| Commands: | Princess Mary's Royal Air Force Nursing Service (1943–48) |
| Battles: | Second World War |
| Awards: | Dame Commander of the Order of the British Empire Royal Red Cross |
Dame Gladys Taylor, (1890 – 11 January 1950) was a British military nurse and nursing administrator who served as Matron-in-Chief of Princess Mary's Royal Air Force Nursing Service from 1943 to 1948.
Born in 1890, Taylor trained at University College Hospital, London. She was appointed a Dame Commander of the Order of the British Empire in the 1949 New Year Honours.