| Gladys Ascanio | |
| Birth Place: | Caracas, Venezuela |
| Birth Date: | 8 February 1941 |
| Birth Name: | Gladys Ascanio Arredondo |
| Eye Color: | Black |
| Hair Color: | Black |
Gladys Ascanio Arredondo (born 8 February 1941) is a Venezuelan model and beauty pageant titleholder who was crowned Miss Venezuela 1960 and was the official representative of Venezuela to the Miss International 1960 pageant held in Long Beach, California, United States, on 12 August 1960, when she classified in the Top 15 semifinalists.