Gastrotheca zeugocystis is a species of frogs in the family Hemiphractidae. It is endemic to Peru and only known from its type locality on the Cordillera de Carpish, Huánuco Region. The specific name zeugocystis refers to the paired brood pouches in this species (from the Greek zeugos, meaning pair, and the Greek kystis, meaning sac).
The holotype, an adult female, measured 37.5mm in snout–vent length; an adult male measured 28.2mm in snout–vent length. The tympanum is visible. The dorsum and head are brown and have small, irregular markings. The dorsal skin is smooth. The iris is reddish brown and has black reticulations. The brood pouches are paired and lateral (most Gastrotheca have a single, dorsal brood pouch).
Gastrotheca zeugocystis inhabits cloud forest at about 2920m (9,580feet) above sea level. Specimens have been found under leaves and under a piece of wood.
Gastrotheca zeugocystis is only known from two individuals. It is threatened by deforestation caused by agricultural expansion and firewood extraction. It is not known from any protected area.