Galactosyl-N-acetylglucosaminylgalactosylglucosyl-ceramide b-1,6-N-acetylglucosaminyltransferase explained

Galactosyl-N-acetylglucosaminylgalactosylglucosyl-ceramide beta-1,6-N-acetylglucosaminyltransferase
Ec Number:	2.4.1.164
Cas Number:	85638-40-0

Galactosyl-N-acetylglucosaminylgalactosylglucosyl-ceramide beta-1,6-N-acetylglucosaminyltransferase (uridine diphosphoacetylglucosamine-acetyllactosaminide beta1->6-acetylglucosaminyltransferase, UDP-N-acetyl-D-glucosamine:D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide beta-1,6-N-acetylglucosaminyltransferase) is an enzyme with systematic name UDP-N-acetyl-D-glucosamine:D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide 6-beta-N-acetylglucosaminyltransferase.^[1] This enzyme catalyses the following chemical reaction

UDP-N-acetyl-D-glucosamine + D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide

\rightleftharpoons UDP + N-acetyl-D-glucosaminyl-(1->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide

This enzyme requires Mn²⁺.

Notes and References

1. Basu M, Basu S . Biosynthesis in vitro of Ii core glycosphingolipids from neolactotetraosylceramide by beta 1-3- and beta 1-6-N-acetylglucosaminyltransferases from mouse T-lymphoma . The Journal of Biological Chemistry . 259 . 20 . 12557–62 . October 1984 . 6238026 .