GalNAc-alpha-(1-4)-GalNAc-alpha-(1-3)-diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase explained

GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase
Ec Number:	2.4.1.292

GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase (PglH) is an enzyme with systematic name UDP-N-acetyl-alpha-D-galactosamine:GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-tritrans,heptacis-undecaprenol 4-alpha-N-acetyl-D-galactosaminyltransferase.^[1] ^[2] ^[3] This enzyme catalyses the following chemical reaction

3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-tritrans,heptacis-undecaprenol

\rightleftharpoons

3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-diNAcBac-PP-tritrans,heptacis-undecaprenol

This enzyme is solated from Campylobacter jejuni.

Notes and References

Glover KJ, Weerapana E, Imperiali B . In vitro assembly of the undecaprenylpyrophosphate-linked heptasaccharide for prokaryotic N-linked glycosylation . Proceedings of the National Academy of Sciences of the United States of America . 102 . 40 . 14255–9 . October 2005 . 16186480 . 1242339 . 10.1073/pnas.0507311102 . 2005PNAS..10214255G . free .
Troutman JM, Imperiali B . Campylobacter jejuni PglH is a single active site processive polymerase that utilizes product inhibition to limit sequential glycosyl transfer reactions . Biochemistry . 48 . 12 . 2807–16 . March 2009 . 19159314 . 2736683 . 10.1021/bi802284d .
Børud B, Viburiene R, Hartley MD, Paulsen BS, Egge-Jacobsen W, Imperiali B, Koomey M . Genetic and molecular analyses reveal an evolutionary trajectory for glycan synthesis in a bacterial protein glycosylation system . Proceedings of the National Academy of Sciences of the United States of America . 108 . 23 . 9643–8 . June 2011 . 21606362 . 3111294 . 10.1073/pnas.1103321108 . 2011PNAS..108.9643B . free .