GRAP explained

GRB2-related adapter protein is a protein that in humans is encoded by the GRAP gene.^{[1] [2] [3]}

This gene encodes a member of the GRB2/Sem5 (C. elegans homolog)/Drk (Drosophila homolog) family. This member functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain.^[3]

Interactions

GRAP has been shown to interact with Linker of activated T cells.^{[4] [5]}

Further reading

• Welsh M, Songyang Z, Frantz JD, Trüb T, Reedquist KA, Karlsson T, Miyazaki M, Cantley LC, Band H, Shoelson SE . Stimulation through the T cell receptor leads to interactions between SHB and several signaling proteins . Oncogene . 16 . 7 . 891–901 . Feb 1998 . 9484780 . 10.1038/sj.onc.1201607 . 28452652 .
• Zhang W, Sloan-Lancaster J, Kitchen J, Trible RP, Samelson LE . LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation . Cell . 92 . 1 . 83–92 . Jan 1998 . 9489702 . 10.1016/S0092-8674(00)80901-0 . 1806525 . free .
• Wenzel J, Sanzenbacher R, Ghadimi M, Lewitzky M, Zhou Q, Kaplan DR, Kabelitz D, Feller SM, Janssen O . Multiple interactions of the cytosolic polyproline region of the CD95 ligand: hints for the reverse signal transduction capacity of a death factor . FEBS Letters . 509 . 2 . 255–62 . Dec 2001 . 11741599 . 10.1016/S0014-5793(01)03174-X . 33084576 .
• Zhao C, Ma H, Bossy-Wetzel E, Lipton SA, Zhang Z, Feng GS . GC-GAP, a Rho family GTPase-activating protein that interacts with signaling adapters Gab1 and Gab2 . The Journal of Biological Chemistry . 278 . 36 . 34641–53 . Sep 2003 . 12819203 . 10.1074/jbc.M304594200 . free .
• Shiraiwa H, Takei M, Yoshikawa T, Azuma T, Kato M, Mitamura K, Ueki T, Kida A, Horie T, Seki N, Sawada S . Detection of Grb-2-related adaptor protein gene (GRAP) and peptide molecule in salivary glands of MRL/lpr mice and patients with Sjögren's syndrome . The Journal of International Medical Research . 32 . 3 . 284–91 . 2004 . 15174222 . 10.1177/147323000403200308 . free .

Notes and References

1. Feng GS, Ouyang YB, Hu DP, Shi ZQ, Gentz R, Ni J . Grap is a novel SH3-SH2-SH3 adaptor protein that couples tyrosine kinases to the Ras pathway . The Journal of Biological Chemistry . 271 . 21 . 12129–32 . May 1996 . 8647802 . 10.1074/jbc.271.21.12129 . free.
2. Trüb T, Frantz JD, Miyazaki M, Band H, Shoelson SE . The role of a lymphoid-restricted, Grb2-like SH3-SH2-SH3 protein in T cell receptor signaling . The Journal of Biological Chemistry . 272 . 2 . 894–902 . Jan 1997 . 8995379 . 10.1074/jbc.272.2.894 . free .
3. Web site: Entrez Gene: GRAP GRB2-related adaptor protein.
4. Zhang W, Sloan-Lancaster J, Kitchen J, Trible RP, Samelson LE . LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation . Cell . 92 . 1 . 83–92 . Jan 1998 . 9489702 . 10.1016/S0092-8674(00)80901-0 . 1806525 . free .
5. Perez-Villar JJ, Whitney GS, Sitnick MT, Dunn RJ, Venkatesan S, O'Day K, Schieven GL, Lin TA, Kanner SB . Phosphorylation of the linker for activation of T-cells by Itk promotes recruitment of Vav . Biochemistry . 41 . 34 . 10732–40 . Aug 2002 . 12186560 . 10.1021/bi025554o .