GOT1 explained

Aspartate aminotransferase, cytoplasmic is an enzyme that in humans is encoded by the GOT1 gene.^{[1] [2]}

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.^[3]

Further reading

• Panteghini M . Aspartate aminotransferase isoenzymes. . Clin. Biochem. . 23 . 4 . 311–9 . 1990 . 2225456 . 10.1016/0009-9120(90)80062-N .
• Doonan S, Barra D, Bossa F . Structural and genetic relationships between cytosolic and mitochondrial isoenzymes. . Int. J. Biochem. . 16 . 12 . 1193–9 . 1985 . 6397370 . 10.1016/0020-711X(84)90216-7 .
• Kamei S, Ohkubo A, Yamanaka M . Apoenzyme of aspartate aminotransferase isozymes in serum and its diagnostic usefullness [sic] for hepatic diseases. . Clin. Chim. Acta . 96 . 1–2 . 97–105 . 1979 . 225064 . 10.1016/0009-8981(79)90058-5 .
• Doyle JM, Schininà ME, Bossa F, Doonan S . The amino acid sequence of cytosolic aspartate aminotransferase from human liver. . Biochem. J. . 270 . 3 . 651–7 . 1990 . 2241899 . 10.1042/bj2700651. 1131781 .
• Pol S, Bousquet-Lemercier B, Pavé-Preux M . Chromosomal localization of human aspartate aminotransferase genes by in situ hybridization. . Hum. Genet. . 83 . 2 . 159–64 . 1989 . 2777255 . 10.1007/BF00286710 . 30300621 . etal.
• Pol S, Bousquet-Lemercier B, Pave-Preux M . Nucleotide sequence and tissue distribution of the human mitochondrial aspartate aminotransferase mRNA. . Biochem. Biophys. Res. Commun. . 157 . 3 . 1309–15 . 1989 . 3207426 . 10.1016/S0006-291X(88)81017-9 . etal.
• Andersson B, Wentland MA, Ricafrente JY . A "double adaptor" method for improved shotgun library construction. . Anal. Biochem. . 236 . 1 . 107–13 . 1996 . 8619474 . 10.1006/abio.1996.0138 . etal.
• Yu W, Andersson B, Worley KC . Large-scale concatenation cDNA sequencing. . Genome Res. . 7 . 4 . 353–8 . 1997 . 9110174 . 10.1101/gr.7.4.353. 139146 . etal.
• Wang CY, Huang YQ, Shi JD . Genetic homogeneity, high-resolution mapping, and mutation analysis of the urofacial (Ochoa) syndrome and exclusion of the glutamate oxaloacetate transaminase gene (GOT1) in the critical region as the disease gene. . Am. J. Med. Genet. . 84 . 5 . 454–9 . 1999 . 10360399 . 10.1002/(SICI)1096-8628(19990611)84:5<454::AID-AJMG9>3.0.CO;2-D . etal.
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Miyake Y, Eguchi H, Shinchi K . Glucose intolerance and serum aminotransferase activities in Japanese men. . J. Hepatol. . 38 . 1 . 18–23 . 2003 . 12480555 . 10.1016/S0168-8278(02)00323-9 . etal.
• Deloukas P, Earthrowl ME, Grafham DV . The DNA sequence and comparative analysis of human chromosome 10. . Nature . 429 . 6990 . 375–81 . 2004 . 15164054 . 10.1038/nature02462 . 2004Natur.429..375D . etal. free .
• Wu KL, Lu SN, Changchien CS . Sequential changes of serum aminotransferase levels in patients with severe acute respiratory syndrome. . Am. J. Trop. Med. Hyg. . 71 . 2 . 125–8 . 2004 . 15306699 . 10.4269/ajtmh.2004.71.125. etal. free .
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Totan A, Greabu M, Totan C, Spinu T . Salivary aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase: possible markers in periodontal diseases? . Clin. Chem. Lab. Med. . 44 . 5 . 612–5 . 2006 . 16681433 . 10.1515/CCLM.2006.096 . 21495180 .
• Dubern B, Girardet JP, Tounian P . Insulin resistance and ferritin as major determinants of abnormal serum aminotransferase in severely obese children. . International Journal of Pediatric Obesity . 1 . 2 . 77–82 . 2007 . 17907318 . 10.1080/17477160600569594.

Notes and References

1. Bousquet-Lemercier B, Pol S, Pave-Preux M, Hanoune J, Barouki R . Properties of human liver cytosolic aspartate aminotransferase mRNAs generated by alternative polyadenylation site selection . Biochemistry . 29 . 22 . 5293–9 . Sep 1990 . 1974457 . 10.1021/bi00474a011 .
2. Web site: Entrez Gene: GOT1 glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1).
3. Web site: Entrez Gene: GOT1 glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1).