GOLT1B explained

Vesicle transport protein GOT1B is a protein that in humans is encoded by the GOLT1B gene.^{[1] [2] [3]}

Got1p is a protein that aides in vesicle transport through the Golgi apparatus of the cell.^[4] Got1p has a calculated mass of 15.4 kDa and consists of 138 residues. It is believed that the protein is a tetra-spanning membrane protein, and is evolutionarily conserved. Homologes of the protein have been found in Caenorhabditis elegans, Plasmodium falciparum, and in mammals. GOT1 is normally associated closely with Sf2p (encoded by sft2), which is another protein of similar function. In vivo, It has been found that the removal of these two proteins results in defects in endosome-Golgi traffic and ER-Golgi traffic. In vitro, the removal of got1 specifically, results in a defect in ER-Golgi transport in relation to vesicle tethering to Golgi membranes. The exact contribution that the protein has towards vesicle tethering is still a mystery, however, it is suspected that Got1p is involved in the release of Ca²⁺ in the Golgi membranes, causing ion channels associated with vesicle tethering to be affected.^[5]

Further reading

• Conchon S, Cao X, Barlowe C, Pelham HR . Got1p and Sft2p: membrane proteins involved in traffic to the Golgi complex . The EMBO Journal . 18 . 14 . 3934–46 . July 1999 . 10406798 . 1171469 . 10.1093/emboj/18.14.3934 .
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A . Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs . Genome Research . 11 . 3 . 422–35 . March 2001 . 11230166 . 311072 . 10.1101/gr.GR1547R .
• Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S . Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing . EMBO Reports . 1 . 3 . 287–92 . September 2000 . 11256614 . 1083732 . 10.1093/embo-reports/kvd058 .
• Matsuda A, Suzuki Y, Honda G, Muramatsu S, Matsuzaki O, Nagano Y, Doi T, Shimotohno K, Harada T, Nishida E, Hayashi H, Sugano S . Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways . Oncogene . 22 . 21 . 3307–18 . May 2003 . 12761501 . 10.1038/sj.onc.1206406 . 38880905 .
• Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A . The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment . Genome Research . 13 . 10 . 2265–70 . October 2003 . 12975309 . 403697 . 10.1101/gr.1293003 .

Notes and References

1. Bourdon V, Naef F, Rao PH, Reuter V, Mok SC, Bosl GJ, Koul S, Murty VV, Kucherlapati RS, Chaganti RS . Genomic and expression analysis of the 12p11-p12 amplicon using EST arrays identifies two novel amplified and overexpressed genes . Cancer Res . 62 . 21 . 6218–23 . Nov 2002 . 12414650 .
2. Lai CH, Chou CY, Ch'ang LY, Liu CS, Lin W . Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics . Genome Res . 10 . 5 . 703–713 . Aug 2000 . 10810093 . 310876 . 10.1101/gr.10.5.703 .
3. Web site: Entrez Gene: GOLT1B golgi transport 1 homolog B (S. cerevisiae).
4. Connerly, PL . How Do Proteins Move Through the Golgi Apparatus? . Nature Education . 3 . 9 . 60 . 2010 .
5. Conchon S, Cao X, Barlowe C, Pelham HR . Got1p and Sft2p: membrane proteins involved in traffic to the Golgi complex . The EMBO Journal . 18 . 14 . 3934–46 . July 1999 . 10406798 . 1171469 . 10.1093/emboj/18.14.3934 .