GDP-L-galactose phosphorylase explained

GDP-L-galactose phosphorylase
Ec Number:	2.7.7.69

GDP-L-galactose phosphorylase (VTC2, VTC5) is an enzyme with systematic name GDP:alpha-L-galactose 1-phosphate guanylyltransferase.^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

\rightleftharpoons alpha-L-galactose 1-phosphate + GDP

The enzyme catalyses a reaction of the Smirnoff-Wheeler pathway.

Notes and References

1. Linster CL, Gomez TA, Christensen KC, Adler LN, Young BD, Brenner C, Clarke SG . Arabidopsis VTC2 encodes a GDP-L-galactose phosphorylase, the last unknown enzyme in the Smirnoff-Wheeler pathway to ascorbic acid in plants . The Journal of Biological Chemistry . 282 . 26 . 18879–85 . June 2007 . 17462988 . 2556065 . 10.1074/jbc.m702094200 . free .
2. Linster CL, Adler LN, Webb K, Christensen KC, Brenner C, Clarke SG . A second GDP-L-galactose phosphorylase in arabidopsis en route to vitamin C. Covalent intermediate and substrate requirements for the conserved reaction . The Journal of Biological Chemistry . 283 . 27 . 18483–92 . July 2008 . 18463094 . 2441562 . 10.1074/jbc.m802594200 . free .
3. Dowdle J, Ishikawa T, Gatzek S, Rolinski S, Smirnoff N . Two genes in Arabidopsis thaliana encoding GDP-L-galactose phosphorylase are required for ascorbate biosynthesis and seedling viability . The Plant Journal . 52 . 4 . 673–89 . November 2007 . 17877701 . 10.1111/j.1365-313x.2007.03266.x . free .
4. Müller-Moulé P . An expression analysis of the ascorbate biosynthesis enzyme VTC2 . Plant Molecular Biology . 68 . 1–2 . 31–41 . September 2008 . 18516687 . 10.1007/s11103-008-9350-4 . 34035343 .