Grancalcin Explained

Grancalcin is a protein that in humans is encoded by the GCA gene.^{[1] [2] [3] [4]}

This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.

Interactions

GCA (gene) has been shown to interact with SRI.

Further reading

• Lim J, Hao T, Shaw C . A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. . Cell . 125 . 4 . 801–14 . 2006 . 16713569 . 10.1016/j.cell.2006.03.032 . 13709685 . etal. free .
• Rual JF, Venkatesan K, Hao T . Towards a proteome-scale map of the human protein-protein interaction network. . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 . etal.
• Hillier LW, Graves TA, Fulton RS . Generation and annotation of the DNA sequences of human chromosomes 2 and 4. . Nature . 434 . 7034 . 724–31 . 2005 . 15815621 . 10.1038/nature03466 . 2005Natur.434..724H . etal. free .
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Jia J, Borregaard N, Lollike K, Cygler M . Structure of Ca(2+)-loaded human grancalcin. . Acta Crystallogr. D . 57 . Pt 12 . 1843–9 . 2002 . 11717497 . 10.1107/S0907444901016511 .
• Lollike K, Johnsen AH, Durussel I . Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner. . J. Biol. Chem. . 276 . 21 . 17762–9 . 2001 . 11279160 . 10.1074/jbc.M100965200 . etal. free .
• Jia J, Han Q, Borregaard N . Crystal structure of human grancalcin, a member of the penta-EF-hand protein family. . J. Mol. Biol. . 300 . 5 . 1271–81 . 2000 . 10903868 . 10.1006/jmbi.2000.3925 . etal.
• Maki M, Narayana SV, Hitomi K . A growing family of the Ca2+-binding proteins with five EF-hand motifs. . 328 . Biochem. J. . (Pt 2) . 718–20 . 1998 . Pt 2 . 9441591 . 1218977 .

Notes and References

1. Boyhan A, Casimir CM, French JK, Teahan CG, Segal AW . Molecular cloning and characterization of grancalcin, a novel EF-hand calcium-binding protein abundant in neutrophils and monocytes . J Biol Chem . 267 . 5 . 2928–33 . Mar 1992 . 10.1016/S0021-9258(19)50675-5 . 1737748 . free .
2. Teahan CG, Totty NF, Segal AW . Isolation and characterization of grancalcin, a novel 28 kDa EF-hand calcium-binding protein from human neutrophils . 286 . Biochem J . (Pt 2) . 2. 549–54 . Oct 1992 . 1530588 . 1132932 . 10.1042/bj2860549.
3. Hansen C, Tarabykina S, la Cour JM, Lollike K, Berchtold MW . The PEF family proteins sorcin and grancalcin interact in vivo and in vitro . FEBS Lett . 545 . 2–3 . 151–4 . Jun 2003 . 12804766 . 10.1016/S0014-5793(03)00518-0 . 42572647 .
4. Web site: Entrez Gene: GCA grancalcin, EF-hand calcium binding protein.