GBA3 explained

Cytosolic beta-glucosidase, also known as cytosolic beta-glucosidase-like protein 1, is a beta-glucosidase enzyme that in humans is encoded by the GBA3 gene.^{[1] [2]}

Function

Cytosolic beta-glucosidase is a predominantly liver enzyme that efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiologic beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides.^[2] GBA3 also has significant neutral glycosylceramidase activity, suggesting that it may be involved in a non-lysosomal catabolic pathway of glucosylceramide metabolism.^[3]

See also

• Closely related enzymes
  • GBA: acid β-glucosidase,
  • GBA2: acid β-glucosidase (bile acid), also

Further reading

• Bolmstedt A, Sjölander S, Hansen JE, Akerblom L, Hemming A, Hu SL, Morein B, Olofsson S . Influence of N-linked glycans in V4-V5 region of human immunodeficiency virus type 1 glycoprotein gp160 on induction of a virus-neutralizing humoral response . J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. . 12 . 3 . 213–20 . July 1996 . 8673525 . 10.1097/00042560-199607000-00001. free .
• Beutler E, Beutler L, West C . Mutations in the gene encoding cytosolic beta-glucosidase in Gaucher disease. . J. Lab. Clin. Med. . 144 . 2 . 65–8 . 2004 . 15322500 . 10.1016/j.lab.2004.03.013 .
• Yahata K, Mori K, Arai H . Molecular cloning and expression of a novel klotho-related protein. . J. Mol. Med. . 78 . 7 . 389–94 . 2000 . 11043382 . 10.1007/s001090000131 . 20851692 . etal.
• Pantophlet R, Wilson IA, Burton DR . Hyperglycosylated mutants of human immunodeficiency virus (HIV) type 1 monomeric gp120 as novel antigens for HIV vaccine design. . J. Virol. . 77 . 10 . 5889–901 . 2003 . 12719582 . 10.1128/JVI.77.10.5889-5901.2003 . 154011.
• Tribolo S, Berrin JG, Kroon PA . The crystal structure of human cytosolic beta-glucosidase unravels the substrate aglycone specificity of a family 1 glycoside hydrolase. . J. Mol. Biol. . 370 . 5 . 964–75 . 2007 . 17555766 . 10.1016/j.jmb.2007.05.034 . etal.
• Noguchi J, Hayashi Y, Baba Y . Crystal structure of the covalent intermediate of human cytosolic beta-glucosidase. . Biochem. Biophys. Res. Commun. . 374 . 3 . 549–52 . 2008 . 18662675 . 10.1016/j.bbrc.2008.07.089 . etal.
• Berrin JG, Czjzek M, Kroon PA . Substrate (aglycone) specificity of human cytosolic beta-glucosidase. . Biochem. J. . 373 . Pt 1 . 41–8 . 2003 . 12667141 . 10.1042/BJ20021876 . 1223474. etal.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library. . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3. etal.
• Wolk T, Schreiber M . N-Glycans in the gp120 V1/V2 domain of the HIV-1 strain NL4-3 are indispensable for viral infectivity and resistance against antibody neutralization. . Med. Microbiol. Immunol. . 195 . 3 . 165–72 . 2006 . 16547752 . 10.1007/s00430-006-0016-z . 31347157 .
• Papandreou MJ, Fenouillet E . Effect of various glycosidase treatments on the resistance of the HIV-1 envelope to degradation. . FEBS Lett. . 406 . 1–2 . 191–5 . 1997 . 9109416 . 10.1016/S0014-5793(97)00273-1. 17660 . free .
• Dekker N, Voorn-Brouwer T, Verhoek M . The cytosolic beta-glucosidase GBA3 does not influence type 1 Gaucher disease manifestation. . Blood Cells Mol. Dis. . 46. 1. 19–26 . 2010 . 20728381 . 10.1016/j.bcmd.2010.07.009 . etal.
• Berrin JG, McLauchlan WR, Needs P . Functional expression of human liver cytosolic beta-glucosidase in Pichia pastoris. Insights into its role in the metabolism of dietary glucosides. . Eur. J. Biochem. . 269 . 1 . 249–58 . 2002 . 11784319 . 10.1046/j.0014-2956.2001.02641.x . etal. free .
• Feizi T, Larkin M . AIDS and glycosylation. . Glycobiology . 1 . 1 . 17–23 . 1990 . 2136376 . 10.1093/glycob/1.1.17.
• Németh K, Plumb GW, Berrin JG . Deglycosylation by small intestinal epithelial cell beta-glucosidases is a critical step in the absorption and metabolism of dietary flavonoid glycosides in humans. . Eur J Nutr . 42 . 1 . 29–42 . 2003 . 12594539 . 10.1007/s00394-003-0397-3 . 20146480 . etal.
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928. etal.
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2002 . 12477932 . 10.1073/pnas.242603899 . 139241. 2002PNAS...9916899M . etal. free .
• Land A, Braakman I . Folding of the human immunodeficiency virus type 1 envelope glycoprotein in the endoplasmic reticulum. . Biochimie . 83 . 8 . 783–90 . 2001 . 11530211 . 10.1016/S0300-9084(01)01314-1. 1874/5091 . 13576808 . free .
• Hart ML, Saifuddin M, Spear GT . Glycosylation inhibitors and neuraminidase enhance human immunodeficiency virus type 1 binding and neutralization by mannose-binding lectin. . J. Gen. Virol. . 84 . Pt 2 . 353–60 . 2003 . 12560567 . 10.1099/vir.0.18734-0. free .

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human Cytosolic beta-glucosidase

Notes and References

1. Web site: Entrez Gene: glucosidase.
2. de Graaf M, van Veen IC, van der Meulen-Muileman IH, Gerritsen WR, Pinedo HM, Haisma HJ . Cloning and characterization of human liver cytosolic beta-glycosidase . Biochem. J. . 356 . Pt 3 . 907–10 . June 2001 . 11389701 . 1221920 . 10.1042/0264-6021:3560907.
3. Hayashi Y, Okino N, Kakuta Y, Shikanai T, Tani M, Narimatsu H, Ito M . Klotho-related protein is a novel cytosolic neutral beta-glycosylceramidase . J. Biol. Chem. . 282 . 42 . 30889–900 . October 2007 . 17595169 . 10.1074/jbc.M700832200 . free .