Fructan beta-(2,6)-fructosidase explained

Fructan beta-(2,6)-fructosidase
Ec Number:	3.2.1.154
Cas Number:	1000597-62-5

Fructan beta-(2,6)-fructosidase (beta-(2-6)-fructan exohydrolase, levanase, 6-FEH, beta-(2,6)-D-fructan fructohydrolase) is an enzyme with systematic name (2->6)-beta-D-fructan fructohydrolase.^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

Hydrolysis of terminal, non-reducing (2->6)-linked beta-D-fructofuranose residues in fructans

The best substrates are the levan-type fructans such as 6-kestotriose [beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl alpha-D-glucopyranoside] and 6,6-kestotetraose [beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl alpha-D-glucopyranoside].

Notes and References

1. Hydrolysis of fructan in grasses: A β-(2-6)-linkage specific fructan-β-fructosidase from stubble of Lolium perenne . Marx SP, Nösberger J, Frehner M . New Phytol. . 1997 . 135 . 279–290 . 10.1046/j.1469-8137.1997.00642.x.
2. Van den Ende W, De Coninck B, Clerens S, Vergauwen R, Van Laere A . Unexpected presence of fructan 6-exohydrolases (6-FEHs) in non-fructan plants: characterization, cloning, mass mapping and functional analysis of a novel "cell-wall invertase-like" specific 6-FEH from sugar beet (Beta vulgaris L.) . The Plant Journal . 36 . 5 . 697–710 . December 2003 . 14617070 . 10.1046/j.1365-313x.2003.01912.x . free .
3. Henson CA, Livingston DP . Purification and characterization of an oat fructan exohydrolase that preferentially hydrolyzes beta-2,6-fructans . Plant Physiology . 110 . 2 . 639–44 . February 1996 . 8742337 . 157760 . 10.1104/pp.110.2.639 .