Formate–tetrahydrofolate ligase explained

Formate—tetrahydrofolate ligase
Ec Number:	6.3.4.3
Cas Number:	9023-66-9
Go Code:	0004329

Symbol:	FTHFS
Formate—tetrahydrofolate ligase
Pfam:	PF01268
Pfam Clan:	CL0023
Interpro:	IPR000559
Prosite:	PDOC00595
Scop:	1fpm

In enzymology, a formate—tetrahydrofolate ligase is an enzyme that catalyzes the chemical reaction

ATP + formate + tetrahydrofolate

\rightleftharpoons ADP + phosphate + 10-formyltetrahydrofolate

The 3 substrates of this enzyme are ATP, formate, and tetrahydrofolate, whereas its 3 products are ADP, phosphate, and 10-formyltetrahydrofolate.

This enzyme belongs to the family of ligases, specifically those forming generic carbon-nitrogen bonds. This enzyme participates in glyoxylate and dicarboxylate metabolism and one carbon pool by folate.

In eukaryotes the FTHFS activity is expressed by a multifunctional enzyme, C-1-tetrahydrofolate synthase (C1-THF synthase), which also catalyses the dehydrogenase and cyclohydrolase activities. Two forms of C1-THF syntheses are known, one is located in the mitochondrial matrix, while the second one is cytoplasmic.^[1] In both forms the FTHFS domain consists of about 600 amino acid residues and is located in the C-terminal section of C1-THF synthase. In prokaryotes FTHFS activity is expressed by a monofunctional homotetrameric enzyme of about 560 amino acid residues.^[2]

Nomenclature

The systematic name of this enzyme class is formate:tetrahydrofolate ligase (ADP-forming). Other names in common use include:

• formyltetrahydrofolate synthetase,
• 10-formyltetrahydrofolate synthetase,
• tetrahydrofolic formylase, and
• tetrahydrofolate formylase.

Examples

Human genes encoding formate-tetrahydrofolate ligases include:

• MTHFD1 – cytoplasmic
• MTHFD1L – mitochondrial

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes,, and .

The crystal structure of N(10)-formyltetrahydrofolate synthetase from Moorella thermoacetica shows that the subunit is composed of three domains organised around three mixed beta-sheets. There are two cavities between adjacent domains. One of them was identified as the nucleotide binding site by homology modelling. The large domain contains a seven-stranded beta-sheet surrounded by helices on both sides. The second domain contains a five-stranded beta-sheet with two alpha-helices packed on one side while the other two are a wall of the active site cavity. The third domain contains a four-stranded beta-sheet forming a half-barrel. The concave side is covered by two helices while the convex side is another wall of the large cavity. Arg 97 is likely involved in formyl phosphate binding. The tetrameric molecule is relatively flat with the shape of the letter X, and the active sites are located at the end of the subunits far from the subunit interface.^[3]

Related enzymes

The reverse reaction converting 10-formyltetrahydrofolate to tetrahydrofolate is performed by formyltetrahydrofolate dehydrogenase.

Further reading

• JAENICKE L, BRODE E . 1961 . [Research on monocarbon compounds. I. The tetrahydrofolate formylase from pigeon liver. Purification and mechanism.] . Biochem. Z. . 334 . 108 - 32 . 13789141 .
• Long CW . Levitzki A . Houston LL . Koshland DE, Jr . 1969 . Subunit structures and interactions of CTP synthetase . Fed. Proc. . 28 . 342 .
• RABINOWITZ JC, PRICER WE . 1962 . Formyltetrahydrofolate synthetase. I. Isolation and crystallization of the enzyme . J. Biol. Chem. . 237 . 9 . 2898 - 902 . 10.1016/S0021-9258(18)60248-0 . 14489619 . free .
• Whiteley HR, Osborn MJ, Huennekens FM . 1959 . Purification and properties of the formate-activating enzyme from Micrococcus aerogenes . J. Biol. Chem. . 234 . 6 . 1538 - 1543 . 10.1016/S0021-9258(18)70046-X . 13654413 . free .

Notes and References

1. Shannon KW, Rabinowitz JC . Isolation and characterization of the Saccharomyces cerevisiae MIS1 gene encoding mitochondrial C1-tetrahydrofolate synthase . J. Biol. Chem. . 263 . 16 . 7717–25 . June 1988 . 10.1016/S0021-9258(18)68558-8 . 2836393 . free .
2. Lovell CR, Przybyla A, Ljungdahl LG . Primary structure of the thermostable formyltetrahydrofolate synthetase from Clostridium thermoaceticum . Biochemistry . 29 . 24 . 5687–94 . June 1990 . 2200509 . 10.1021/bi00476a007 .
3. Radfar R, Shin R, Sheldrick GM, Minor W, Lovell CR, Odom JD, Dunlap RB, Lebioda L . The crystal structure of N(10)-formyltetrahydrofolate synthetase from Moorella thermoacetica . Biochemistry . 39 . 14 . 3920–6 . April 2000 . 10747779 . 10.1021/bi992790z .