| State: | FL |
| Type: | SR |
| Route: | 163 |
| Maint: | FDOT |
| Length Mi: | 2.194 |
| Length Round: | 3 |
| Decommissioned: | yes |
| Direction A: | South |
| Direction B: | North |
State Road 163 (SR 163) was a 2.194adj=midNaNadj=mid north–south state highway in Duval County. It traveled in a question mark shape, completely within the northeastern part of Jacksonville.
SR 163 began at an intersection with SR 105 (Heckscher Drive), near the Jacksonville Zoo and Gardens. It headed to the north before curving to the northeast. The road curved back to the north, then to the northwest and west, before meeting its northern terminus, an intersection with U.S. Route 17 (US 17; North Main Street) at the southeastern edge of the Anheuser-Busch brewery. This intersection also marks the eastern terminus of SR 104 (Dunn Avenue).