| Florence Duperval Guillaume | |
| Office: | Prime Minister of Haiti |
| President: | Michel Martelly |
| Term Start: | 20 December 2014 |
| Term End: | 16 January 2015 |
| Predecessor: | Laurent Lamothe |
| Successor: | Evans Paul |
| Birth Place: | Port-au-Prince, Haiti |
| Party: | Independent |
Florence Duperval Guillaume (pronounced as /fr/) is a Haitian politician who was Haiti's Minister of Public Health and Population, and was Acting Prime Minister of Haiti from 20 December 2014 to 16 January 2015.[1]