Fibrolase Explained

Fibrolase
Ec Number:	3.4.24.72
Cas Number:	116036-70-5

Fibrolase (fibrinolytic proteinase, Agkistrodon contortrix contortrix metalloproteinase, Agkistrodon contortrix contortrix venom metalloproteinase) is an enzyme.^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

Hydrolysis of -Ala¹⁴-Leu- in insulin B chain and -Lys⁴¹³-Leu- in alpha-chain of fibrinogen

This enzyme is present in the venom of the southern copperhead snake (Agkistrodon contortrix contortrix).

Notes and References

1. Retzios AD, Markland FS . A direct-acting fibrinolytic enzyme from the venom of Agkistrodon contortrix contortrix: effects on various components of the human blood coagulation and fibrinolysis systems . Thrombosis Research . 52 . 6 . 541–52 . December 1988 . 3232124 . 10.1016/0049-3848(88)90127-2 .
2. Guan AL, Retzios AD, Henderson GN, Markland FS . Purification and characterization of a fibrinolytic enzyme from venom of the southern copperhead snake (Agkistrodon contortrix contortrix) . Archives of Biochemistry and Biophysics . 289 . 2 . 197–207 . September 1991 . 1898066 . 10.1016/0003-9861(91)90462-r .
3. Randolph A, Chamberlain SH, Chu HL, Retzios AD, Markland FS, Masiarz FR . Amino acid sequence of fibrolase, a direct-acting fibrinolytic enzyme from Agkistrodon contortrix contortrix venom . Protein Science . 1 . 5 . 590–600 . May 1992 . 1304358 . 2142229 . 10.1002/pro.5560010505 .
4. Loayza SL, Trikha M, Markland FS, Riquelme P, Kuo J . Resolution of isoforms of natural and recombinant fibrolase, the fibrinolytic enzyme from Agkistrodon contortrix contortrix snake venom, and comparison of their EDTA sensitivities . Journal of Chromatography B . 662 . 2 . 227–43 . December 1994 . 7719479 . 10.1016/0378-4347(94)00202-9 .
5. Retzios AD, Markland FS . Fibrinolytic enzymes from the venoms of Agkistrodon contortrix contortrix and Crotalus basiliscus basiliscus: cleavage site specificity towards the alpha-chain of fibrin . Thrombosis Research . 74 . 4 . 355–67 . May 1994 . 8085237 . 10.1016/0049-3848(94)90151-1 .